Kyoto Daiichi Kagaku Co., Ltd.

Amano Pharmaceutical Co., Ltd.

Nippon Zeon Company, Ltd.

Other

Daicel Chemical Industries, Ltd.

Suntory Limited

Nitto Kagaku Kogyo Kabushiki Kaisha

Yoshiki Tani

Hideaki Yamada

Yasuyoshi Sakai

Nobuo Kato

Sakayu Shimizu

Masayuki Yagi

Fumiyo Funatsu

Kaori Ishimaru

Toshikatsu Sakai

Hiroshi Fukuya

Shozo Shiozaki

Kimiyasu Isobe

Hiroaki Yamamoto

Nobuyuki Yoshida

Yuji Shibano

Haruyo Hatanaka

Hiroto Kondo

Satoshi Yonehara

Kanzo Yamashita

Yoshikazu Izumi

Keiko Onodera

Satoshi Cyousa

The present invention provides a fructosylamine oxidase which is obtainable by culturing, and purifying two types of fructosylamine oxidase (FAO) with different substrate specificities from the culture, and which is useful in the measurement of amadori compounds.

Fructosylamine oxidase

The objective of the present invention is to produce a novel (R)-2,3-butanediol dehydrogenase useful for the production of ketones, alcohols, and particularly optically active vicinal diols. It has been found that produces novel (R)-2,3-butanediol dehydrogenase that shows the high activity and high stereoselectivity. Further, polynucleotide encoding this (R)-2,3-butanediol dehydrogenase was cloned, and the nucleotide sequence thereof was determined. The expression of the glycerol dehydrogenase was carried out in heterologous microorganisms.

(R)-2,3-butanediol dehydrogenase

Fructosyl amino acid oxidase, process for producing the same, and method

A novel fructosyl amino acid oxidase derived from genus Fusarium or Gibberella, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme, a process for producing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine as a substrate of a fructosyl amino acid oxidase, which is useful for screening and/or culturing a microorganism capable of producing the enzyme, and a medium containing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine for screening and/or culturing a microorganism capable of producing a fructosyl amino acid oxidase is provided.

Fructosyl amino acid oxidase and process for producing the same

The present invention provides a recombinant protein which shows fructosyl amino acid oxidase activity, a DNA encoding the same, an expression vector containing the DNA, a transformant transformed by the expression vector, and the method of preparing recombinant fructosyl amino acid oxidase by culturing the resultant transformant, and the recombinant fructosyl amino acid oxidase thus obtained.

Recombinant fructosyl amino acid oxidase

A novel fructosyl amino acid oxidase derived from genus Penicillium, which is active on both of fructosyl lysine and fructosyl valine, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme is provided.

The present invention relates to an expression vector which is induced by methanol and/or glycerol utilizing an alcohol oxidase gene of a methylotrophic yeast; a recombinant methylotrophic yeast containing said vector and capable of accumulating a significantly high amount of an expression product of a heterologous gene; and a method for producing useful products utilizing said recombinant. The expression vector is constructed by utilizing the promoter and terminator of an alcohol oxidase gene of methylotrophic yeast. Further, a significantly high amount of adenylate kinase, cytochrome C552 or peroxidase is produced by using said expression vector.

Vector having promoter that is inducible by methanol and/or glycerol

A novel fructosyl amino acid oxidase derived from genus Fusarium, which is active on both of fructosyl lysine and fructosyl valine, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme is provided.

Fructosyl amino acid oxidase

Process for producing S-adenosyl-L-homocysteine, which comprises reacting adenosine with homocysteine by contacting them in an aqueous medium in the presence of cells or treated cells of a microorganism of a specified genus having the ability to synthesize S-adenosyl-L-homocysteine from adenosine and homocysteine, and collecting the S-adenosyl-L-homocysteine synthesized.

Process for producing S-adenosyl-L-homocysteine

Process for biological preparation of amides

In a process which comprises cultivating a micro-organism having the ability to produce a biotin-vitamer in a culture medium in the presence of a biotin-vitamer precursor thereby to produce the biotin-vitamer and accumulate it in the culture medium, the improvement wherein the biotin-vitamer precursor is added to the culture medium after the microbial cells have been grown.

Process for producing biotin-vitamers

A yeast culture containing at least 10% by weight, based on the dry cell, of S-adenosyl methionine; and a process for producing S-adenosyl methionine, which comprises cultivating a yeast having the ability to produce S-adenosyl methionine in a liquid culture medium containing methionine to accumulate at least 10% by weight, based on the dry yeast cells, of S-adenosyl methionine in the yeast cells, separating the yeast cells from the culture medium, and thereafter obtaining S-adenosyl methionine in a stable form from the yeast cells.

Yeast culture containing S-adenosyl methionine in high concentrations,

A novel polyamine oxidase, polyamine oxidase PC-3, takes an important role participating in decomposition and metabolism and polyamines such as spermidine and spermine.

Microbial polyamide oxidase PC-3 and process for preparation thereof

A novel polyamine oxidase, polyamine oxidase AT-1, takes an important role participating in decomposition and metabolism of polyamines such as spermidine and spermine.

Growing community of inventors

Kyoto, Japan

Yoshiki Tani