Fuji Photo Film Company, Limited

Fujifilm Corporation

Yoshiki Sakaino

Hitoshi Shimizu

Kentaro Nakamura

Hideaki Tanaka

Kaoru Terashima

Yoshihide Iwaki

Kentarou Nakamura

Yoshikazu Amano

Fumio Kawamoto

Yukio Sudo

Yoshihiko Abe

Shohei Yoshida

Osamu Seshimoto

Toshihiro Mori

Hideyuki Karaki

Akira Wakabayashi

Yasutomo Goto

Kazuya Kawasaki

Isao Ikuhara

Toshihisa Ito

Hitomi Ito

Bo Yang

Ichizou Toya

An object of the present invention is to provide a microchip whereby, when chemical analysis is conducted, a specimen liquid can be directly collected and weighed without using any collecting and weighing devices. The present invention provides a microchip for analyzing liquid samples, which has a measuring structure for weighing and collecting a given amount of a specimen liquid within a range of 0.05 to 10 &#x3bc;l from an excessive amount of the specimen liquid which was introduced in the chip, wherein the measuring structure is located at the upstream side of an analysis element for analyzing a target substance in the specimen liquid inside the microchip.

Microchip and analysis method using the same

The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

Immunoassay element

A humoral testing apparatus in which a measuring light is irradiated to a reagent area forming a color as a result of a reaction and an optical density of the reagent area is detected by a detecting operation of light intensity of the reflected light. The humoral testing apparatus enables a humoral test to be performed accurately in cases where nonuniformity occurs with the reaction of a reagent with a humoral sample within the reagent area, or in cases where fine dust is present within each detecting spot in the reagent areas. The light reflected from the reagent areas is detected with a two-dimensional photodetector. The independent light intensity detecting operations are performed with respect to the subareas of the reagent area of a reagent layer. A photo detection signal S, which represents the intensity of the reflected light is statistically processed with a signal processing section 51.

Humoral testing apparatus

A liquid filtering instrumentaccording to the present invention achieves filtered liquid by pressure-reduced filtration, and it comprises a filter memberin which a liquid filteris accommodated, and a holder memberfor stocking filtered liquid passed through the liquid filter, wherein the filter memberand the holder memberare formed in capable of being fitted so as to be kept substantially air-tight and water-tight under pressure reduced state.

Liquid filtering instrument and dry type analysis device

An optical measuring apparatus comprises: an illuminating section that illuminates a first object with a first light; a light changing section that changes an intensity of the first light illuminating the first object; a light receiving device that receives a second light transmitted through or reflected from the first object; and an output section that outputs a measurement result according to i) a state of the light changing section and ii) an amount of the second light received by the light receiving device. In addition, an optical measuring apparatus comprises: the illuminating section; the light receiving device; a light receiving time changing section that changes a light receiving time of the light receiving device; and an output section that outputs a measurement result according to i) the light receiving time and ii) an amount of the second light received by the light receiving device.

Optical measuring apparatus and optical measuring method

A blood testing unit is provided with a closed vessel having an outer vessel body and an inner vessel body, and a reagent layer is accommodated in the closed vessel. Reagents which react with a blood sample are supported on the reagent layer. The closed vessel is comprised such that at least either one of the outer vessel body and the inner vessel body is provided with an aperture, through which air is capable of being introduced from the exterior to the interior of the one vessel body, and the one vessel body is provided with a sealing member for closing the aperture.

Humoral testing unit

A blood testing unit comprises a closed vessel having a blood introducing section formed at a certain area of the closed vessel. A blood constituent separating section is located within the closed vessel to separate plasma and/or serum from the blood sample introduced through the blood introducing section into the closed vessel. A reagent layer is located within the closed vessel, such that the reagent layer can be seen from the exterior. The reagent layer comprises a region for spreading the plasma and/or the serum, which has been separated by the blood constituent separating section from the blood sample, and a reagent supporte donor in the spreading region, the reagent undergoing a reaction with the plasma and/or the serum and forming a color as a result of the reaction.

Blood testing unit and blood testing method and apparatus

A plurality of different kinds of reagents are supported at different positions on a reagent layer. A humoral testing apparatus irradiates measuring light which has a wavelength adapted to one of the plurality of the reagent areas which forms a color, and detects the intensity of the light reflected from the reagent areas with the photodetector with respect to each of the reagents, and thus detects the optical densities of the reagent areas.

Humoral testing unit and a humoral testing apparatus

This invention relates to a dry analytical element using a water-soluble indicator which improves measuring accuracy, which comprises a water-impermeable support, a water-permeable layer and a spreading layer having a function to spread a liquid uniformly, wherein a water-soluble indicator for colorimetry is incorporated into the s preading layer.

Dry analytical element using water-soluble indicator for colorimetry

A dry analytical element for quantitatively analyzing creatine kinase is composed of a support and a creatine kinase-detecting agent layer. The creatine kinase-detecting agent layer contains creatine phosphate and adenosine diphosphate which react with each other to give adenosine triphosphate in the presence of creatine kinase, a buffer compound having a sulfonic acid group which keeps the layer in the range of pH 5.5 to pH 8.5 for the formation of adenosine triphosphate, and an indicator composition which reacts with the formed adenosine triphosphate to give a spectroscopically detectable compound. An analytical element for analysis of creatine kinase MB isozyme further contains in the layer an antibody which specifically inhibits activity of M sub-unit of creatine kinase.

Sulfonic acid group buffered dry analytical element for quantitative

A method of producing a photographic polyester laminate support having a laminate of layers of at least two kinds of polyesters can provide low formation of scratches in a camera and a curl in the film's width direction. In the support, the glass transition temperature (Tg) of each polyester of the laminate is within the range of from 90.degree. C. to 200.degree. C. and the laminate is subjected to heat-fixing in a temperature range as defined by formula (1):

Method of producing a photographic polyester laminate support

Disclosed is a silver halide photographic material provided with at least one silver halide emulsion layer on a support and wound in a roll form, wherein the above support is subjected to a heat treatment until a heat amount in an endothermic peak which appears including a glass transition temperature becomes 100 to 1,000 mcal/g, and the support is of biaxially oriented polyester having a loss elastic modulus of 0.01 to 0.1, the glass transition temperature of 50.degree. to 200.degree. C., a Young's modulus of 530 to 2,000 kg/mm.sup.2, a breaking elongation of 60 to 200%, and a ratio of the refraction indexes in a film face direction and a thickness direction of 1.10 to 1.22.

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Asaka, Japan

Yoshiki Sakaino