Toyo Boseki Kabushiki Kaisha

Yoshikazu Ishida

Yoshihisa Kawamura

Yoshiaki Nishiya

Masao Kitabayashi

Tadayuki Imanaka

Toshihiro Kuroita

Hideyuki Komatsubara

Bunsei Kawakami

Masanori Oka

An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DXEXXXH sequence (D: aspartic acid, E: glutamic acid, H: histidine, X, X, Xand X: any amino acid) consisting of DXE sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3'-5&#x2032; exonuclease activity, histidine(H) has been replaced by another amino acid.

Modified thermostable DNA polymerase

The invention provides methods, kits, and compositions for enhancing synthesis of DNA involving a carboxylate ion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions. The invention further provides a thermostable DNA polymerase-related factor derived fromspecies, which has an activity to promote the DNA synthesis activity of DNA polymerase or which binds to DNA polymerase.

Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof

Growing community of inventors

Tsuruga, Japan

Yoshikazu Ishida