Japan Science and Technology Agency

Japan Science and Technology Corporation

Yoshihisa Inoue

Victor V Borovkov

Juha Lintuluoto

Takehiko Wada

Kimoon Kim

Narayanan Selvapalam

Mikhail Rekharsky

Yong Ho Ko

Tatsuo Nakagawa

Guy A Hembury

Asao Nakamura

Kido Okamoto

Hideo Nishino

A cryostat includes: a casing having an inlet port and exit port; a cell housing provided in the casing; a temperature controller for adjusting the temperature of the cell; a first optical path tube for guiding a light beam from the inlet port of the casing to the cell housing; a second optical path tube for guiding the light beam having passed through the cell housing to the exit port of the casing; first and second optical windows disposed at openings, exposed to the outside, of the first and second optical path tubes, respectively; and sealing materials having a water vapor transmission rate of 30000 cc&#xb7;cm&#xb7;mm&#xb7;sec&#xb7;cm Hg&#xd7;10or lower, disposed at the peripheries of the first and second optical windows to seal the first and second optical path tubes.

Cryostat

The present invention provides a method for quantifying a peptide compound having a phenylalanine residue at the N-terminal, comprising measuring the content of heat generated by mixing 1) a peptide compound having a phenylalanine residue at the N-terminal, 2) cucurbit[7]uril, and 3) a metal ion in a solution.

Method for determining quantity of peptide compound having phenylalanine residue at N-terminus

The present invention provides a method for determining in a pharmaceutical test formulation the presence or absence of a peptide compound PYYrepresented by the following amino acid sequence: H-Ile-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Asn-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-X (SEQ ID NO: 1), wherein X is OH or a carboxy acid-protecting group, the method comprising (1) preparing a solution by mixing the pharmaceutical test formulation with cucurbit[7]uril in a solvent; and (2) thermally analyzing the solution prepared in Step (1).

[object Object]

The present invention provides a simple, highly sensitive, and highly precise method for determining the absolute configuration of a chiral compound such as a diamine or an amino alcohol. The present invention is directed to a method for determining the absolute configuration of an asymmetric carbon of a chiral compound on the basis of the sign of the Cotton effect by analyzing a solution containing the chiral compound and a porphyrin dimer by circular dichroism spectroscopy.

Method for determination of absolute configuration of chiral compounds

The present invention relates to a reagent for determining the absolute configuration of a chiral compound containing as an active ingredient a metalloporphyrin dimer, wherein the metalloporphyrin dimer has an alkaline-earth metal ion as a central metal and has a carbon chain-crosslinked structure in which at least one of the two porphyrin rings has a substituent bulkier than methyl at least one of the second carbon atoms from a carbon atom bonded to the crosslinking carbon chain along the outer periphery of the porphyrin ring and a method for determining the absolute configuration of an asymmetric carbon atom of the chiral compound using the reagent.

Reagent for determining the absolute configuration of chiral compound and determination method

An antisense molecule which acts on both directions of the inhibition and expression of a gene function and is capable of on-off switching of a gene function appropriately depending on the external factors (orientation controlling factors); and a method for reversibly controlling the expression of a gene function by using the antisense molecule. Such an antisense molecule, which has at least one sugar-base moiety consisting of sugar and a purine or pyrimidine base bonded thereto via a glucoside bond, can bind to a mRNA/gene and/or dissociate therefrom under the orientation control of the base moiety in the molecule by the orientation controlling factors.

Antisense molecules and method of controlling expression of gene function by using the same

The absolute configuration of a chiral compound is determined by (i) coordinating the chiral compound to a metalloporphyrin having a carbon chain-crosslinked porphyrin dimer structure in which one of the two porphyrin rings has at least one ethyl or substituent bulkier than ethyl at at least one of the second peripheral carbon atoms from the carbon atom at the carbon chain crosslink site, and (ii) analyzing the resultant coordination compound by circular dichroism spectrophotometry to determine the absolute configuration of the asymmetric carbon based on the sign of the Cotton effect. The chiral compound has an asymmetric carbon bonded to a basic group capable of coordinating to the metal of the other porphyrin ring of the metalloporphyrin dimer or an asymmetric carbon atom adjacent to the carbon atom bonded to the basic group.

Method of determining absolute configuration of chiral compound

Novel nucleoside derivatives represented by the following general formula (1):wherein X is(are) the same or different and each represents a pyrimidine or purine base or a derivative thereof, Y and Y' are the same or different and each represents at least one amino acid or amino acid derivative selected from the group consisting of serine, threonine, ornithine, aspartic acid, glutamic acid, lysine, arginine, cysteine, methionine, &#x3b4;-hydroxylysine, N-aminoethylglycine, N-aminoethylserine, N-aminoethyllysine, N-aminoethylornithine, N-aminoethylaspartic acid, N-aminoethylglutamic acid, homoglutamic acid, &#x3b2;-thiocarbonylaspartic acid, &#x3b3;-thiocarbonylglutamic acid, and &#x3b4;-thiocarbonylhomoglutamic acid, Rrepresents a hydrogen atom or a hydroxyl group, A represents a single bond or a carbonyl or thiocarbonyl group, 1 is an integer of 0 to 5, and n is an integer of 1 to 100.

Nucleoside derivatives

Method for synthesizing absolute asymmetry

A process for producing a metalloporphyrin containing a transition metal inserted into the porphyrin ring which comprises dissolving a porphyrin compound and a transition metal salt each in an independent solvent, combining the solutions, and carrying out the reaction in the presence of a basic substance. By this process a metalloporphyrin containing a transition metal inserted into the porphyrin ring can be synthesized easily under mild conditions and with high selectivity.

Growing community of inventors

Toyonaka, Japan

Yoshihisa Inoue