Ajinomoto Co., Ltd.

Yoshihiro Usuda

Kazuhiko Matsui

Shinichi Sugimoto

Yousuke Nishio

Hisashi Yasueda

Shintaro Iwatani

Osamu Kurahashi

Takuji Ueda

Tomoko Suzuki

Shigeo Suzuki

Hisashi Kawasaki

Yoshinori Tajima

Eiichiro Kimura

Yasuhiro Mihara

Mika Moriya

Keita Fukui

Hisao Ito

Hiroshi Matsui

Tsuyoshi Nakamatsu

Yoshihiko Hara

Jun Yamazaki

Yoshio Kawahara

Hiroshi Izui

Yury Ivanovich Kozlov

Masakazu Sugimoto

Natalia Viktorovna Stoynova

Yuta Nakai

Nobuharu Tsujimoto

Yoko Asakura

Akira Imaizumi

Tatyana Abramovna Yampolskaya

Veronika Aleksandrovna Kotliarova

Katsuaki Sato

Leonid Romanovich Ptitsyn

Hidetaka Doi

Stephen Van Dien

Chie Koseki

Yuichiro Tsuji

Kazue Kawamura

Shuhei Hashiro

Yasushi Hoshino

Yuri Nagai

Seizaburo Shiraga

Masaru Terashita

Megumi Shimaoka

Masahiro Kakehi

Yosuke Nishio

Natalia Sergeevna Eremina

Akiko Tanaka

Takashi Utagawa

Irina Borisovna Altman

Kazuyuki Hayashi

Yusuke Hagiwara

Akiko Matsudaira

Kazuho Ikeo

Takashi Gojobori

Chizu Abe

Yoji Nakamura

Yutaka Kawarabayashi

Yumi Hino

Eiichi Hori

Noriko Murayama

Yuri Masumitsu

Yukiko Tabira

Olga Nikolaevna Mokhova

Stephen c/o Ajinmoto Co Inc Van Dien

A method for producing an L-amino acid is provided. An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid-producing ability, wherein the bacterium has been modified so that the activity of aconitase is increased, or the activities of aconitase and acetaldehyde dehydrogenase are increased, in a medium, and collecting the L-amino acid from the medium or cells of the bacterium.

Method for producing L-amino acids

An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

Method for producing an L-glutamic acid and L-aspartic acid using a recombinant microorganism having enhanced expression of a ybjL protein

An L-amino acid is produced by culturing a microorganism of the family Enterobacteriaceae which has the ability to produce an L-amino acid and which has been modified so as to increase the expression of the evgA gene, the gadE gene, and/or the ydeO gene. These genes encode a transcription factor involved in the EvgAS two-component system regulon. The culture takes place in a medium, and the L-amino acid is collected from the medium or cells.

L-amino acid-producing bacterium and a method for producing an L-amino acid

A method for efficiently producing an L-amino acid utilizing a bacterium belonging to the family Enterobacteriaceae from a fatty acid or an alcohol such as glycerol as a raw material is provided. A bacterium belonging to the family Enterobacteriaceae which is able to produce L-amino acid and harbors an RpsA protein which has a mutation such that the native aspartic acid residue at positionis replaced with another amino acid residue is used. This bacterium is cultured in a medium containing a carbon source selected from a fatty acid and an alcohol, and the produced L-amino acid is collected from the medium.

Mutant RpsA gene and method for producing L-amino acid

A method for producing an L-amino acid includes culturing a bacterium which belongs to the family Enterobacteriaceae and has an L-amino acid-producing ability in a medium containing a carbon source selected from a fatty acid and an alcohol, and collecting the L-amino acid from the medium. A bacterium which has been subjected to a modification including at least one of enhancement of oxyS gene expression, enhancement of fixABC gene expression, and combination thereof, is used as the bacterium, or a substance that reduces intracellular hydrogen peroxide concentration of the bacterium is added to the medium.

Method for producing an L-amino acid

A method for producing an L-amino acid which includes the steps of culturing a bacterium belonging to the family Enterobacteriaceae and having an L-amino acid producing ability in a medium to produce and accumulate an L-amino acid in the medium, and collecting the L-amino acid from the medium, wherein the bacterium has been modified so that an activity or activities of one or two or more enzymes of the arginine succinyltransferase pathway, such as arginine succinyltransferase, succinylarginine dihydrolase, succinylornithine aminotransferase, succinylglutamate-semialdehyde dehydrogenase, and succinylglutamate desuccinylase, is/are decreased.

Method for producing an L-amino acid in a cultured  bacterium having reduced activity of the arginine succinyltransferase pathway

An L-amino acid is produced by culturing a bacterium having an L-amino acid-producing ability in a medium containing a processed product of a microalga which promotes production and accumulation of the L-amino acid by the bacterium. The process product is produced by disrupting the culture of the microalga, and/or extracting the culture of the microalga, or fractionating the culture of the microalga or the disrupted culture. The processed product contains a mixture of organic substances produced by the microalga, a hydrolysate of the disrupted microalga culture, and/or an extract or fractionation product of the microalga culture. The processed product can also contain a saccarification product of starch or a hydrolysate of fats and oils. The bacterium is cultured to produce and accumulate the L-amino acid in culture, and the L-amino acid is collected from the culture.

An L-amino acid is produced by culturing a bacterium of the Enterobacteriaceae family which has an L-amino acid-producing ability in a medium containing fatty acids as the carbon source, particularly fatty acids which have been subjected to emulsification or homogenization, to thereby produce and accumulate the L-amino acid in a culture medium; and collecting the L-amino acid from the culture medium.

An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.

Method of producing L-amino acid

A method for intracellular metabolic flux analysis comprising (a) culturing cells in a medium not containing any isotope-labeled substrate to a target phase of the metabolic flux analysis, (b) adding an isotope-labeled substrate to the medium, and continuing culture and collecting samples from the medium in time course, (c) measuring isotope distribution in an intracellular metabolite contained in the samples collected in time course, (d) performing a regression analysis for measured data and calculating an isotope distribution ratio in a steady state, and (e) analyzing a metabolic flux of the cultured cells by using the calculated isotope distribution ratio.

Intracellular metabolic flux analysis method using substrate labeled with isotope

The present invention relates to a method for modifying a property of a protein. The present invention also relates to a method for producing a protein which has a modified property, and a method for producing a microorganism which has a modified property. The present invention is useful in the field of microbial industrial production and the like.

Method for modifying a property of a protein

An L-amino acid can be produced by culturing a bacterium which belongs to the Enterobacteriaceae family, and has an enhanced ability to use a fatty acid. The bacterium is capable of producing the L-amino acid in a culture medium containing a fatty acid or a hydrolysate of an oil-and-fat as a carbon source, thereby producing and accumulating the L-amino acid in a culture.

Method for production of L-amino acid

A bacterium which belongs to the Enterobacteriaceae family and has an ability to produce an L-amino acid such as L-lysine, L-threonine and L-tryptophan and is modified to enhance glutamic acid decarboxylase activity is cultured in a medium to produce and accumulate the L-amino acid in the medium or cells of the bacterium. Then, the L-amino acid is collected from the medium or the cells.

A bacterium is described which belongs to the Enterobacteriaceae family, and has an ability to produce an L-amino acid, such as L-glutamic acid, L-arginine and L-threonine. The bacterium is modified so that the activity of a protein encoded by ydcI gene is decreased, thereby producing and accumulating the L-amino acid selected from L-glutamic acid, L-arginine, and L-threonine in the culture medium or cells of the bacterium when cultured in a culture medium. Subsequently, the L-amino acid is collected from the culture medium or the bacterium.

Bacterium capable of producing L-amino acid and method for producing L-amino acid

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Kawasaki, Japan

Yoshihiro Usuda