Toyo Boseki Kabushiki Kaisha

Yoshihiro Soya

Yoshihisa Kawamura

Shigenori Emi

Yutaka Takarada

Shigeaki Nishii

Kazuhiro Matsui

Takuya Ishibashi

Shinichi Teschima

A method for identifying a nucleotide polymorphism, the method comprising hybridizing a labeled oligonucleotide for a wild type or a mutant type to a nucleic acid containing a specific nucleotide polymorphic site in a sample; allowing a nucleic acid-specific label to act thereon; detecting an interaction between the label of the oligonucleotide and the nucleic acid-specific label; and identifying a nucleotide polymorphism, wherein the identification utilizes a difference due to the nucleotide polymorphism in conditions under which the hybridized oligonucleotide is separated into a single strand.

Method for identifying nucleotide polymorphism

The present invention provides a method capable of simultaneous processing of plural test samples for the receptor binding property of a chemical substance, which does not require immobilization of the receptor or a special device, and a reagent to be used for this method. That is a method for assaying the receptor binding property of an assay target substance is provided, the method comprising the steps of (a) competitively reacting a known concentration of a ligand and the assay target substance with a known concentration of the receptor in a solution, (b) measuring, without physically removing the ligand bound with the receptor prior to the assay, the amount of a free ligand in the solution using one or more antibodies against the ligand, and (c) determining the receptor binding property of the assay target substance using the amount of the free ligand as an index.

Method for assaying receptor binding property and reagent for the assay

There is disclosed a composition for measurement of potassium ion concentration, which contains a) pyruvate kinase, b) glycerol kinase, c) glycerol-3-phosphate oxidase, d) peroxidase, e) a chromogen of reduced type, f) adenosine diphosphate or a salt thereof, and g) phosphoenolpyruvic acid or a salt thereof. Also disclosed is a composition for elimination of ammonium ions, which contains a) glutamate dehydrogenase, b) glucose dehydrogenase, and c) nicotinamide adenine dinucleotide of reduced type (NADH) or nicotinamide adenine dinucleotide phosphate of reduced type (NADPH).

Composition for measurement of potassium ion concentration and

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Tsuruga, Japan

Yoshihiro Soya