Tokyo University of Agriculture and Technology

Yokogawa Electric Corporation

Yoshihiro Ozeki

Akiyo Yamada

Nobuhiro Sasaki

Yutaka Abe

Tomoyuki Taguchi

Haruka Nakamura

Kazuo Nagasawa

Takeyuki Mogi

Hitoshi Wake

Masayuki Tera

Yuki Matsuba

Satoko Akatsuka

Satoko c/o Japan Women's University Akatsuka

There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100&#xb0; C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100&#xb0; C. with the vessel held in a sealed state.

Nucleic acid extraction method

An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A): wherein Ris independently selected from hydrogen, or Calkyl, Calkenyl, and Calkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO, and SO, n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a &#x3b2; bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.

Glycosyltransferase, novel glycosyltransferase gene, and novel sugar donor compound

It is intended to provide a gene encoding a protein having an activity of improving a tolerance to an environmental stress such as salt, moisture, or heat stress, in particular, a tolerance to water stress; a protein having an activity of improving a tolerance to an environmental stress; a transgenic plant having an enhanced tolerance to an environmental stress, and so on. A halophyte, which has a strong activity of improving a tolerance to water stress and can grow even in a dry area with much salt accumulation, was used to search genes participating in the salt tolerance ofby a functional screening method using angene expression system, with the aim of isolating genes (cDNAs) participating in the salt-tolerance mechanism of. As a result, it was found out that an arabinogalactan protein consisting of 427 amino acids and having partial homology to a Fasciclin-like arabinogalactan protein ofhas a function of enhancing salt tolerance, water stress tolerance (sorbitol tolerance), and heat tolerance.

Arabinogalactan protein having activity of improving tolerance to heat or moisture stress

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Fuchu, Japan

Yoshihiro Ozeki