National Institute of Advanced Industrial Science and Technology

Toyo Boseki Kabushiki Kaisha

Other

Atto Corporation

Yoshihiro Ohmiya

Chun Wu

Yoshihiro Nakajima

Satoru Ohgiya

Kosei Kawasaki

Vadim R Viviani

Shigeaki Nishii

Hidehiro Kubota

Hidefumi Akiyama

Rie Yasuno

Seiji Ito

Yasuo Mitani

Masahiro Shimogawara

Yasushi Ohyama

Emiko Ashitaka

Masahiro Yoshita

Vadim Viviani

Tomomi Asai

Kaneo Mori

Yousuke Morita

The present invention provides a luminescent enzyme protein comprising the amino acid sequence represented by SEQ ID NO: 2, and a mutant enzyme thereof.

Nucleic acid encoding a luminescent enzyme protein

A light-emitting element, in which a light whose emission angle distribution is one of Lambert's emission law or uniform Isotropic emission, is extracted from a light extraction opening window, and an in-plane distribution of a light intensity on a light extraction surface of the light extraction opening window is uniform, and which can be used as a reference light source when measuring an absolute light quantity of a weak light emitted from a luminous body which is a measurement object.

Light emitting element, reference light source and method for observing luminous body

The present invention relates to a method for producingluciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted withluciferase, and biotin-labeledluciferase wherein a sugar chain inluciferase has been biotinylated.

[object Object]

The present invention provides a gene construct encoding pH insensitive luciferase for visualizing intracellular information, wherein an intracellular expression activity is higher compared with a gene construct of luciferase derived from a firefly.

Luciferase gene optimized for use in imaging of intracellular luminescence

The present invention is one gene construct or a combination of two gene constructs or expression vectors incorporating aluciferase gene and a copepod luciferase under the control of distinct promoters. These gene constructs and expression vectors are useful for making a mammalian cell incorporating theluciferase gene and the copepod luciferase to be capable of stably expressed and extracellularly secreted under the control of the distinct promoters.

Two secretory luciferases

The present invention relates to a method for producing marine ostracod crustacean luciferin or a derivative thereof represented by a general formula (4), characterized by reacting a compound represented by a general formula (2) with a compound represented by a general formula (3): wherein R, R, R, R, Yand Zare the same as defined in the specification.

Luciferin luminescent substrate of marine ostracod crustacean and method for production thereof

An object of the present invention is to provide a mutant luciferase having luciferase activity with an altered emission spectrum. A specific amino acid residue(s) is substituted in a luciferase derived fromand then the resulting mutant luciferase having luciferase activity with an emission spectrum differing from that of the wild-type luciferase is screened for.

Mutant luciferase

An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.

Visible to near-infrared light probe using energy transfer between luciferase and an organic dye via a sugar chain

Method for producing substituted imidazo[1,2-A]pyrazines of (s)-1-(3-(2-sec-butyl-6-(1h-indol-3-yl)-3-oxo-3,7-dihydroimadazo[1,2-a]pyrazin-8-yl)propyl)guanidine

The present invention relates to a stabilization composition of coelenterazine or an analog thereof, a kit, a method for measuring a coelenterazine-based biological luminescence, containing coelenterazine or the analog thereof and an antioxidant.

Stabilizing composition and stabilizing method of coelenterazine solution for high-throughput measurement of luciferase activity

A gene construct incorporating any of at least two luciferase genes which emit lights with different colors using an identical substrate such that the gene can be stably expressed in mammalian cells.

Multiple gene transcription activity assay system

A secretory or membrane-binding chimeric protein composed of an energy-generating protein and an energy-receiving protein linked one another wherein energy transfer can arise between the energy-generating protein and the energy-receiving protein.

Nucleic acids encoding membrane-binding proteins and methods of using same

Isolated nucleic acid molecules which code for luciferases able to produce the green bioluminescence ofand red bioluminescence ofare described. The nucleic acid molecules and the luciferases encoded thereby can be used in applications such as diagnostic methods and molecular biology tools.

Growing community of inventors

Ikeda, Japan

Yoshihiro Ohmiya