Olympus Corporation

Akita University

Governor of Akita Prefecture

Yoshihiro Minamiya

Tomoaki Ueda

Yoichi Akagami

Junichi Ogawa

Hiroshi Ikeda

Masami Kagaya

Yoshihisa Katayose

Reijiro Saito

Ryuta Nakamura

Shuichi Kamada

Hiroshi Nanjo

Acceleration and automation of immunohistochemical staining are achieved as follows: an automatic electric field immunohistochemical staining apparatus is configured to provided with a sample mounting unit, a solution supply unit, an electric field stifling unit, and a washing unit; the sample mounting unit on which a glass substrate with a tissue specimen fixed thereto is mounted and the electric field stirring unit that includes an upper electrode are operated in coordination to activate an antigen in the tissue specimen; the sample mounting unit and the solution supply unit containing various solutions are operated in coordination to supply a primary-antibody-containing solution to the tissue specimen; the sample mounting unit and the electric field stirring unit are operated in coordination to perform an antigen-antibody reaction of the antigen in the tissue specimen and a primary antibody; and the electric field stirring unit and the washing unit are operated in coordination.

Apparatus for automatic electric field immunohistochemical staining and method for automatic electric field immunohistochemical staining

The apparatus of the present invention comprises: an excitation device which excites the measurement site with a local direct-current or alternating-current magnetic field, and a plurality of magnetic field detection devices which are disposed in proximity to each other to measure the magnetic field component that is perpendicular to the exciting magnetic field. A magnetic fluid that possesses paramagnetism is injected from the outside into a substance in which a fluid can flow, and the local magnetic field gradient is then measured while the injected magnetic fluid is excited by the application of a direct-current or alternating-current magnetic field from the outside, thus measuring the presence or absence of distortion of the local magnetic field distribution arising from the fact that the specific magnetic permeability of the magnetic fluid injected into the substance is higher than that of the surrounding substance, so that sites where large amount of the magnetic fluid has accumulated are identified in a non-invasive manner without being affected by external magnetic noise.

Magnetic fluid detection method and magnetic fluid detection apparatus

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Akita, Japan

Yoshihiro Minamiya