Nitto-denko Corporation

Nagoya University

Nippon Zeon Company, Ltd.

Yoshihiro Hayakawa

Kenjiro Mori

Kenjiro Minomi

Eri Maeta

Masaki Tsukamoto

Tatsuya Konishi

Yasuyoshi Chino

Noritaka Suzuki

Shinichiro Tahara

Hisatoyo Kato

Mamoru Uchiyama

Ryuji Nojiri

The invention provides a universal linker capable of synthesizing nucleic acid having a hydroxy group at the 3' terminal, a universal support carrying the linker, and a synthesis method of nucleic acid using the universal support. The linker contains a compound represented by the formula wherein each symbol is as defined in the specification.

Linker and support for solid phase synthesis of nucleic acid

The invention provides a universal linker capable of synthesizing nucleic acid having a phosphate group at the 3' terminal, a universal support carrying the linker, and a synthesis method of nucleic acid using the universal support. The linker for solid phase synthesis of nucleic acid contains a compound represented by at least one of the following formulae wherein each symbol is as defined in the specification.

Phosphoramidite compounds of the general formula ##STR1## wherein each of R.sub.1 and R.sub.2 represents a hydroxyl group having a protective group, or the group --OR.sub.4, R.sub.3 represents a hydrogen atom, a hydroxyl group having a protective group, or the group --OR.sub.4, R.sub.4 represents the group ##STR2## X represents a secondary amino group, R.sub.5 represents an allylic residue or a protective group capable of being split off by beta-cleavage, and B.sup.AOC represents a nucleoside base residue in which the amino or imino group is protected with an allyloxycarbonyl-type residue, with the proviso that only one of R.sub.1, R.sub.2 and R.sub.3 represents the group--OR.sub.4. The compounds can be produced by reacting a nucleoside represented by the general formula ##STR3## wherein each of R.sub.1 ' and R.sub.2 ' represents a hydroxyl group which may have a protective group, R.sub.3 ' represents a hydrogen atom, or a hydroxyl group which may have a protective group, and B.sup.AOC is as defined, with the proviso that only one of R.sub.1 ', R.sub.2 'and R.sub.3 ' is a hydroxyl group, with a phosphoramide compound represented by the general formula ##STR4## wherein X and R.sub.5 are as defined, and Y represents a secondary amino group or a halogen atom.

Phosphoramidite compounds and process for production thereof

Growing community of inventors

Ichinomiya, Japan

Yoshihiro Hayakawa