Toyo Boseki Kabushiki Kaisha

Other

Texas Tech University System

Toyobo Co., Ltd.

Yoshihiko Maekawa

Yoshihisa Kasukabe

Randy Dale Allen

Susumu Nishiguchi

Koichi Fujisawa

Izumi Ihara

Hakuji Matsumoto

Tairo Oshima

Hitoshi Sakashita

Shin-Ichiro Nishimura

Bunsei Kawakami

Kuriko Yamada

Song Ping

Nobuhiro Katsuragi

Cotton plants of the genuswith improved cotton fiber characteristics are disclosed. The cotton plant has an expression cassette containing a gene coding for an enzyme selected from the group consisting of endoxyloglucan transferase, catalase and peroxidase so that the gene is expressed in cotton fiber cells to improve the cotton fiber characteristics. Also disclosed is a method for producing cotton fibers with improved fiber characteristics from these cotton plants.

Cotton plants with improved cotton fiber characteristics and method for producing cotton fibers from these cotton plants

Cotton plants of the genus Gossypium with improved cotton fiber characteristics are disclosed. The cotton plant has an expression cassette containing a gene coding for an enzyme selected from the group consisting of endoxyloglucan transferase, catalase and peroxidase so that the gene is expressed in cotton fiber cells to improve the cotton fiber characteristics. Also disclosed is a method for producing cotton fibers with improved fiber characteristics from these cotton plants.

Methods are disclosed to achieve an improvement in the characteristics and yield of cotton fibers. In one method, a cotton plant of the genus Gossypium in seed form or in growth stage is treated with a brassinosteroid. In another method, an ovule culture is prepared from a cotton plant of the genus Gossypium in a brassinosteroid-containing liquid medium. Cotton fibers with improved fiber characteristics are obtained from the cotton bolls of the treated plant or from the cultured ovules. Also disclosed are a method for inducing specific genes expression in a cotton plant to produce cotton fibers with improved fiber characteristics by treatment with a brassinosteroid, and a cotton plant produced by this method; as well as a novel gene capable of changing the degree of its expression found in the ovules of a cotton plant treated with a brassinosteroid, a gene capable of hybridizing with this novel gene, a recombinant plasmid containing this novel gene, a transformant containing this recombinant plasmid, and the like.

Production of cotton fiber with improved fiber characteristics

Novel plant promoters isolated from cotton fiber tissues are provided. The plant promoters are located upstream cotton plant genes KC03, KC18, KC22, and Gh3, all of which are expressed in cotton fibers, and contain (a) DNA having the nucleotide sequence of SEQ ID NO:1, 6, 11, or 16. Also provided are plant expression vectors prepared by introducing the plant promoters into appropriate vectors; transformed plant cells prepared by introducing the plant expression vectors into host plant cells; transformed plants regenerated from the transformed plant cells; transformed plant seeds obtained from the transformed plants; and processes for producing transformed plants from the plant seeds.

Cotton plant promoters

Production of cotton fibers with improved fiber characteristics

Cotton fiber tissue-specific genes disclosed herein are specifically expressed in a cotton fiber tissue at the stage of cotton fiber elongation. One of the genes was first derived from a cotton plant of the genus Gossypium and found to change the degree of its expression by treatment with a brassinosteroid.

Cotton fiber tissue-specific genes

A method for producing glycoconjugate, which comprises the steps of:

Method for producing glycoconjugates

Cotton fiber tissue-specific genes disclosed herein are specifically expressed in a cotton fiber tissue at the stage of cotton fiber elongation. One of the genes was first derived from a cotton plant of the genus Gossypium and found to change the degree of its expression by treatment with a brassinosteroid. These novel genes are useful for the production of cotton fibers with improved characteristics.

Production of cotton fibers with improved fiber characteristics by

A DNA polymerase from Thermus thermophilus which retains at least 60% of its activity when placed at pH 8.0 and 85.degree. C. for 2 hours is disclosed. The DNA polymerase has a pH optimum of about 8.0, a temperature optimum of about 75.degree. C., a mass of about 85 to 95 kDa and an optimal saline concentration of about 20-60 mM NaCl.

Thermostable DNA polymerase from Thermus thermophilus HB-8

A DNA polymerase with excellent thermostability is provided. The DNA polymerase retains at least about 60% of its activity when placed at pH 8.0 for 2 hours at 85.degree. C.

Method of producing a thermostable DNA polymerase from Thermus

Thermostable DNA polymerase thermus thermophilus and a method of

A recombinant vector comprising an incorporated chromosome DNA fragment containing a PvuI restriction enconuclease gene derived from Proteus vulgaris, a host transformed with the recombinant vector and a method of producing PvuI restriction endonuclease characterized in that the transformed host is cultivated and PvuI restriction endonuclease is harvested from the resulting culture. Since the host transformed with the recombinant vector of the present invention produces PvuI alone, it is unnecessary to remove PvuII in the purification process for PvuI, and further, since its productivity for nonspecific DNase is lower in comparison with conventional producer bacteria, DNase can be removed easily, making the production of PvuI easy.

Growing community of inventors

Ohtsu, Japan

Yoshihiko Maekawa