Ajinomoto Co., Ltd.

Yoshihiko Hara

Hiroshi Izui

Hisao Ito

Kazuhiko Matsui

Mika Moriya

Yury Ivanovich Kozlov

Konstantin Vyacheslavovich Rybak

Ekaterina Aleksandrovna Slivinskaya

Akito Chinen

Hisashi Yasueda

Eiji Ono

Joanna Yosifovna Katashkina

Mikhail Markovich Gusyatiner

Elvira Borisovna Voroshilova

Takuji Ueda

Keita Fukui

Tatyana Viktorovna Leonova

Seiko Hirano

Vera Georgievna Doroshenko

Sergei Vladimirovich Mashko

Elena Vitalievna Klyachko

Ekaterina Alekseevna Savrasova

Larisa Gotlibovna Airikh

Valeriy Zavenovich Akhverdian

Tsuyoshi Nakamatsu

Takahiro Asano

Rie Takikawa

Yasuyuki Watanabe

Yoshihiro Usuda

Jun Nakamura

Gen Nonaka

Yoshinori Tajima

Kazuhiro Takumi

Kazue Kawamura

Masakazu Sato

Kazuteru Yamada

Naoki Akiyoshi

Hiromi Noguchi

Ranko Nishi

Daiki Yahagi

Suguru Yamakami

A method for producing a target substance is provided. A target substance is produced by culturing a coryneform bacterium, which is able to produce a target substance, and which also has an improved ability to assimilate xylose as a result of the introduction of a mutation into a coding region and/or an expression control region of the NCgl2954 gene on the chromosome of the bacterium. The culture is conducted in a medium containing xylose, and the target substance can be collected from the medium.

Method for producing target substance

A method for producing an objective substance is provided. An objective substance is produced by culturing a microorganism which has been modified so that the activity of a dicarboxylic acid exporter protein is reduced in a medium, and collecting the objective substance from the medium.

Method for manufacturing useful substance

A method for producing a dicarboxylic acid is provided. A dicarboxylic acid is produced by culturing a bacterium having a dicarboxylic acid-producing ability, which has been modified so that the expression of one or more of the yeeA gene, ynfM gene, yjjP gene, and yjjB gene is increased, in a medium, and collecting the dicarboxylic acid from the medium.

Method for producing dicarboxylic acid

An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.

Method for producing an L-glutamic acid and L-aspartic acid using a recombinant microorganism having enhanced expression of a ybjL protein

The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

Use of phosphoketolase for producing useful metabolites

There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

Method for producing L-amino acids using bacteria of the enterobacteriaceae family

The present invention describes an L-glutamic acid-producing bacterium which belongs to the genusor, wherein the bacterium has been modified by gene recombination to inactivate the rpoS gene. A method is also described for culturing the bacterium in a medium to cause accumulation of L-glutamic acid in the medium, and collecting L-glutamic acid from the medium.

L-glutamic acid producing bacterium and a method for producing L-glutamic acid

An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has an L-amino acid-producing ability and inherently has a native activity of a glucose dehydrogenase that uses pyrroloquinoline quinone as a coenzyme, but has been modified so that the activity of the glucose dehydrogenase is reduced, in a medium, and collecting the L-amino acid from the medium.

L-amino acid-producing microorganism and a method for producing an L-amino acid

L-Glutamic acid is produced by culturing in a liquid culture medium a microorganism belonging to the genusorand having an ability to produce L-glutamic acid, which increases in an activity of enzyme catalyzing a reaction for L-glutamic acid biosynthesis or which decreases in or is deficient in an activity of an enzyme catalyzing a reaction branching from a pathway for L-glutamic acid biosynthesis and producing a compound other than L-glutamic acid, and collecting produced L-glutamic acid from the culture medium.

L-glutamic acid-producing bacterium and method for producing L-glutamic acid

A microorganism which has an L-amino acid producing ability and has been modified so that succinate dehydrogenase activity and &#x3b1;-ketoglutarate dehydrogenase activity are decreased is cultured in a medium to produce and accumulate an L-amino acid in the medium or cells of the microorganism, and the L-amino acid is collected from the medium or cells to produce the L-amino acid.

Method for producing an L-amino acid

A microorganism is provided which can metabolize a carbon source at a specific pH in a liquid medium containing L-glutamic acid at a saturation concentration and the carbon source, and which has ability to accumulate L-glutamic acid in an amount exceeding the amount corresponding to the saturation concentration in the liquid medium at the pH. Also provided is a method for producing L-glutamic acid by fermentation, which comprises culturing the microorganism in a liquid medium of which pH is adjusted to a pH at which L-glutamic acid is precipitated, to produce and accumulate L-glutamic acid and precipitate L-glutamic acid in the medium.

Method for producing L-glutamic acid by fermentation accompanied by precipitation

A microorganism belonging to the family Enterobacteriaceae, which has an L-amino acid-producing ability and has been modified so that the kdp system is enhanced, is cultured in a medium to produce and accumulate an L-amino acid in the medium or cells of the microorganism, and the L-amino acid is collected from the medium or cells to produce the L-amino acid.

L-amino acid producing microorganism and a method for producing an L-amino acid

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Kawasaki, Japan

Yoshihiko Hara