Fuji Photo Film Company, Limited

Fujifilm Corporation

Yoshihiko Abe

Yoshihiko Makino

Kaoru Terashima

Masashi Ogawa

Shigeori Takenaka

Makoto Takagi

Kenichi Yamashita

Osamu Seshimoto

Hideaki Tanaka

Yoshihiro Seto

Yoshiki Sakaino

Shigeru Tezuka

Yasushi Fujisaki

Seiichi Watanabe

Nobuhito Masuda

Fumio Sugaya

Hiroko Inomata

Hikaru Tsuruta

Yoshiyuki Doi

An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived fromorsp. and cholesterol oxidase derived fromsp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.

Method for measuring high density lipoprotein cholesterol

An optical measuring apparatus comprises: an illuminating section that illuminates a first object with a first light; a light changing section that changes an intensity of the first light illuminating the first object; a light receiving device that receives a second light transmitted through or reflected from the first object; and an output section that outputs a measurement result according to i) a state of the light changing section and ii) an amount of the second light received by the light receiving device. In addition, an optical measuring apparatus comprises: the illuminating section; the light receiving device; a light receiving time changing section that changes a light receiving time of the light receiving device; and an output section that outputs a measurement result according to i) the light receiving time and ii) an amount of the second light received by the light receiving device.

Optical measuring apparatus and optical measuring method

It is an object of the present invention to provide a nucleic acid fragment-fixed electrode wherein a probe nucleic acid fragment is fixed on the electrode stably and in an amount-controlled manner. The present invention provides a nucleic acid fragment-fixed electrode wherein a nucleic acid fragment is fixed on the surface of a multi-component self-assembled monolayer of two or more different components which is formed on the electrode, by a covalent bond via a bifunctional linking molecule.

Nucleic acid fragment-fixed electrode and its use

A method of analyzing a target nucleic acid fragment is provided that is capable of being performed simply and promptly, by anyone, using a compact device without requirement of specific technique or complex operation. The method comprises timely detection of a double-stranded nucleic acid fragment formed during the polymerase extension reaction due to a specific base sequence of the target nucleic acid fragment as increase of an electrochemical response under the existence of an electrochemically active intercalator; and the kit utilizes the method.

Method and kit for analyzing target nucleic acid fragment

A method of detecting nucleic acid fragments in plural samples is performed by the steps of: attaching an electroconductive label to nucleic acid fragments in one sample and attaching a different electroconductive label to nucleic acid fragments in another sample; preparing a mixture of these samples; spotting the mixture on an electroconductive microarray having plural electrodes onto which probe molecules complementary to the nucleic acid fragments are fixed, so that hybridization between the nucleic acid fragments and the probe molecules on the electroconductive microarray can proceed to form hybrid structures; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the former label and detecting on the electrode an electric current; applying to the electrode an electric potential corresponding to the oxidation-reduction potential of the latter label and detecting on the electrode an electric current; and comparing the electric current detected in the former detecting procedure and that detected in the latter detecting procedure.

Method for detecting nucleic acids

A DNA chip (or PNA chip) composed of a solid carrier and plural DNA fragments (or PNA fragments) fixed onto is the solid carrier at each one end, wherein a plurality of short chain spacer molecules having a hydrophilic moiety at each one end are fixed at each another end onto a surface of the solid carrier having no DNA fragments (or no PNA fragments) on its surface is effective for high sensitive quantitative analysis of a nucleic acid fragment complementary to the DNA fragment (or PNA fragment).

DNA chip, PNA chip, and their preparation methods

Protection of partial complementary nucleic acid fragment using a electroconductive chip and intercalator

A dry analytical element for quantitatively analyzing creatine kinase MB isozyme is composed of a support and a creatine kinase MB isozyme-detecting agent layer. The detecting layer contains an antibody which specifically inhibits activity of M sub-unit of creatine kinase, creatine phosphate and adenosine diphosphate which react with each other to give adenosine triphosphate in the presence of creatine kinase MB isozyme, a buffer compound which keeps the layer in the range of pH 5.5 to pH 8.5 for the formation of adenosine triphosphate, and an indicator composition which reacts with the formed adenosine triphosphate to give a spectroscopically detectable compound, said indicator composition having hexokinase, nicotinamide-adenine dinucleotide or its phosphate in an oxidized form, and glucose-6-phosphate dehydrogenase but not containing glucose in such amount as to give the spectroscopically detectable compound.

Glucose free, stable dry analytical element for quantitative analysis of

A dry analytical element for quantitatively analyzing creatine kinase is composed of a support and a creatine kinase-detecting agent layer. The creatine kinase-detecting agent layer contains creatine phosphate and adenosine diphosphate which react with each other to give adenosine triphosphate in the presence of creatine kinase, a buffer compound having a sulfonic acid group which keeps the layer in the range of pH 5.5 to pH 8.5 for the formation of adenosine triphosphate, and an indicator composition which reacts with the formed adenosine triphosphate to give a spectroscopically detectable compound. An analytical element for analysis of creatine kinase MB isozyme further contains in the layer an antibody which specifically inhibits activity of M sub-unit of creatine kinase.

Sulfonic acid group buffered dry analytical element for quantitative

A chemical analysis film chip is prepared by integrally laminating a reagent layer and a spreading layer on a support sheet in this order, or by integrally laminating a water absorbing layer having a hydrophilic polymer as a major component and a porous outermost layer containing therein a reagent on a support sheet in this order, or by superposing a porous layer containing therein a reagent on a support sheet.

Frameless chemical analysis film clip

A cartridge for storing a plurality of stacked chips having an exit for taking out the end dry analytical film chip one by one which are pushed toward the exit, and a flexible engager for inhibiting the discharge of the end chip. The exit and the engager are provided at an end portion of a side wall of the casing, the opening being provided at an end of the casing, and the flexible engager has a strength capable of releasing the end chip from the conveyor but is also capable of holding the second end, wherein the exit and the engager are provided at an end portion of a side wall of the casing, the opening being provided at an end of the casing. The engager is capable of holding the second end chip, or wherein the exit and the engager are provided at an end portion of the casing, and the engager is a non-flexible engager for releasing the end chip by its deformation occurring by the pull of the conveyor through the second end through the release of the lowermost end chip, and the exit also functions as the opening. The cartridge has a compact size and ejects only one chip while preventing the simultaneous ejection of two chips.

Cartridge for storing dry analytical film chips

A chemical analysis element cartridge includes a box-like cartridge body in which a plurality of chemical analysis elements are stacked and are taken out through an element take-out port formed at one end of the cartridge body. A pressing member is slidably received in the cartridge body and is brought into contact with the stack of the elements on the side remote from the element take-out port to push the stack of the elements toward the element take-out port. The cartridge body is provided at least in one side wall thereof with a slit which extends in the direction of stack of the elements and the pressing member is provided with an external force receiving portion which is laterally interlocked with an urging member disposed outside the cartridge body through the slit and receives a force for urging the pressing member toward the element take-out port from the urging member. The pressing member is interlocked with an engagement member so that the pressing member and the engagement member are moved toward the element take-out port together with each other when the force from the urging member is acting on the pressing member and the engagement member permits the pressing member to be displaced away from the element take-out port relative to the engagement member when the force from the urging member is released.

Chemical analysis element cartridge

A chemical analysis film cartridge having a box-like casing body with opposing first and second end walls and in which dry frameless chemical analysis film chips are stored in a stack between the end walls. A first opening is formed in a side wall of the casing body near the first end wall so that only the film chip nearest the first end wall can be removed from the casing body through the first opening. A second opening in the first end wall permits a suction pad to hold the film chip nearest to the first end wall and to take the film chip out of the casing body through the first opening. The width of the first opening is larger than the thickness of one film chip and smaller than double the thickness of one film chip. The suction pad is retracted so that the film chip abuts against the edge of the second opening on opposite sides of the suction pad, and then the suction pad is further retracted to pull the central portion of the film chip into the second opening, thereby reshaping the film chip into a predetermined warped-shape conforming to the shape of the first opening.

Chemical analysis film cartridge

A chemical analysis film cartridge with a box-like casing body having first and second end walls opposed to each other between which a plurality of dry frameless chemical analysis film chips are stored in a stack. A first opening is formed in a side wall of the casing body near the first end wall so that only the film chip nearest the first end wall can be taken out of the casing body through the first opening by moving the film chip in a direction perpendicular to the stack. A second openings which permits a suction pad to hold the film chip nearest the first end wall and to remove it from the casing body through the first openings is formed in the first end wall. The width of the first opening is larger than the thickness of one film chip and smaller than double the thickness of one film chip over at least a portion of the first opening. The suction pad is retracted with the film chip so that the film chip abuts against the edge of the second opening on opposite sides of the suction pads and then the suction pad is further retracted to pull the central portion of the film chip in this state into the second opening, thereby reshaping the film chip into a predetermined warped-shape conforming to the shape of the first opening.

Growing community of inventors

Asaka, Japan

Yoshihiko Abe