Kaneka Corporation

Kanegafuchi Kagaku Kogyo Kabushiki Kaisha

Yoshifumi Yanagida

Yasuyoshi Ueda

Tadashi Moroshima

Yoshihide Fuse

Koichi Kinoshita

Satomi Takahashi

Kiyoshi Watanabe

Takehisa Ohashi

Kenji Inoue

Nobuo Nagashima

Fumio Osakada

Tatsuya Honda

Shingo Matsumoto

Yasuhiro Saka

Kazuhiko Yamada

Namito Yoshio

Yoshio Namito

The present invention provides a method to efficiently degrade nucleic acids, which result in a viscosity increase of the solution thereof on the occasion of decomposition or solubilization of microbial cells, in an easy and simple manner in the step of recovering various useful substance produced by a microorganism, and a use thereof. The product recovery method of the present invention make the product recovery from within microbial cells with ease under relatively mild conditions, because, by bringing living microbial cells into contact with a little amount of hypochlorous acid or a salt thereof, autodigestion of nucleic acids is induced and following decomposition of microbial cells or viscosity increase of the solution thereof after dissolution is inhibited. The method of the present invention is particularly preferred in recovering polyhydroxyalkanoates, which are biodegradable polymers, from microbial cells.

Method for degradation of nucleic acids and use thereof

Process for preparing pharmacologically acceptable salt of N-(1(S)-ethoxycarbonyl-3-phenylpropyl)-L-alanyl-amino acid

Process for preparing pharmacologically acceptable salt of n-(1(s)-ethoxycarbonyl-3-phenylpropyl)-L-alanyl-amino acid

Process for the preparation of 3-hydroxytetrahydrofuran

Process for preparing pharmacologically acceptable salt of N-(1(S)-ethoxycarbonyl-3-phenylpropyl)-L-alanyl amino acids

Process for producing N2-(1(S)-carboxy-3-phenylpropyl)-L-lysyl-L-proline

A process for simply and easily crystallizing a maleate salt of N-(1(S)-ethoxycarbonyl-3-phenylpropyl)-L-alanyl-L-proline (enalapril) having a high quality out of an aqueous liquid, which comprises mixing an aqueous liquid having a pH of not less than 4 and containing N-(1(S)-ethoxycarbonyl-3-phenylpropyl)-L-alanyl-L-proline, maleic acid and a base with an amount of an acid sufficient to convert substantially all the base into a neutral salt.

Method for crystallizing maleic acid salt of N-(1(S)-ethoxycarbonyl-3-phenylpropyl)-L-alanyl-L-proline

A process for preparing a trans-.beta.-aroylacrylic ester which comprises reacting an aryl methyl ketone and an alkyl acetal of alkyl glyoxylate in the presence of an acid catalyst with heating. This process is excellent in operability, safety and ecomony and therefore is industrially advantageous process.

Process for preparing trans-.beta.-aroylacrylic ester

A process for preparing trans-.beta.-benzoylacrylic acid ester having the general formula (I): ##STR1## wherein R is alkyl group or aralkyl group, which comprises dealcoholizing .beta.-benzoyl-.alpha.-alkoxypropionic acid ester having the general formula (II): ##STR2## wherein R is above, in the presence of an acid catalyst to give trans-.beta.-benzoylacrylic acid ester having the general formula (I). According to the process of the present invention, the by-product (II) produced in the esterification reaction of .beta.-benzoylacrylic acid (III) with the alcohol (V) by the dehydration reaction can be converted into the compound (I) by the dealcoholization reaction in the presence of the acid catalyst and thus trans-.beta.-benzoylacrylic acid ester (I) with a high purity can be produced in an industrially advantageous manner.

Process for preparing trans-.beta.-benzoylacrylic acid ester

An inorganic acid salt of N-[1(S)-ethyoxycarbonyl-3-phenylpropyl]-L-alanylchloride having the formula (I): ##STR1## A process for preparing an inorganic acid salt of N-[1(S)-ethoxycarbonyl-3-phenylpropyl]-L-alanylchloride having the formula (I) ##STR2## which comprises reacting an inorganic acid salt of N-[1(S)-ethoxycarbonyl-3-phenylpropyl]-L-alanine with phosphorous pentachloride in an inactive organic solvent. According to the present invention, ACEI can be economically and easily prepared.

Inorganic acid salt of

A process for preparing N-[1(S)-ethoxycarbonyl-3-phenylpropyl]-L-alanyl-L-proline, which comprises reacting N-carboxyanhydride of N-[1(S)-ethoxycarbonyl-3-phenylpropyl]-L-alanine with L-proline in the presence of a base. According to the process, enalapril can be prepared economically, easily and effectively.

Process for preparing

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Kakogawa, Japan

Yoshifumi Yanagida