International Reagents Corporation

Amano Pharmaceutical Co., Ltd.

Yoshifumi Watazu

Yasushi Shirahase

Koji Kishi

Tsutomu Kakuyama

Kenji Isshiki

By a process comprising inhibiting LDH,, LDH,, LDH,and LDH,in a sample with a protease in the presence of a protein-denaturating agent and then determining LDH,remaining uninhibited, LDH,in the sample is easily determined.

LDH1 assay

A method for assaying biological specimens for substances contained in the components of each specimen by the use of one or more kinds of calixarenes; and a reagent comprising one or more kinds of calixarenes. The method utilizes complexes formed by calixarenes and the components of biological specimens, and makes it possible to assay biological specimens for substances contained in the components of each specimen, for example, for cholesterol contained in high-density lipoprotein (HDL), without preliminary separation from the other components of the biological specimen. The method can be conducted by easy and simple operations and lessen measurement errors or problems caused by man, and permits continuous measurement with general-purpose automatic analyzing apparatuses and multi-channel measurement together with other test items.

Method for assaying biological specimens for substances contained in the

A glutamic-oxaloacetic transaminase (GOT) isozyme assay, which comprises selectively inhibiting s-GOT in a sample such as serum or plasma by proteinase K, optionally an excess amount of proteinase K by a protease inhibitor and then determining m-GOT remaining intact, is described.

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Hyogo, Japan

Yoshifumi Watazu