Tosoh Corporation

Yoshifumi Kurihara

Takahiko Ishiguro

Toshinori Hayashi

Juichi Saitoh

A method for detecting fluorescence from a liquid sample housed in a transparent or translucent sample container includes providing a sample container in a sample holder, which is opaque except for a sample container introduction opening and incoming and outgoing openings for excitation light; layering a liquid sample and a shielding liquid unmixable therewith to prevent external light from entering through the sample container introduction opening; introducing excitation light from such a direction that the excitation light can irradiate the liquid sample before irradiating the shielding liquid; and detecting fluorescence, which emits in a direction of avoiding absorption by the shielding liquid.

Fluorescence detection method capable of making measurement under external light

A fluorescence detection apparatus comprising: a sample holder for fixing and holding sample vessels on a circular arc; a partition plate being joined to drive means for rotation on the center of the circular arc; an excitation light source; excitation light optical means; and fluorescence optical means containing a light guide being fixed on the partition plate for rotation integrally and a photosensor. The photosensor is mechanically discontinued from the drive means and fixedly placed. The fluorescence signal emission end of the light guide is placed facing the photosensor on the rotation center axis and the partition plate and the parts fixed thereto are rotated integrally, whereby fluorescence detection of the samples arranged on the circular arc is repeated in order.

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Ebina, Japan

Yoshifumi Kurihara