Director of National Food Research Institute, Ministry of Agriculture,

Bio-Oriented Technology Research Advancement Institution

Yoshifumi Ito

Akihiro Hino

Truong Nam Hai

Hirohito Miura

Seung-Moon Park

The present invention provides a complementary DNA for a rice chitinase having a lytic activity against moulds and a bacteria, a complementary DNA for a rice chitinase encoding a protein comprising an amino acid sequence described in SEQ ID NO: 1 in SEQUENCE LISTING, a plasmid vector containing said complementary DNA and a transformant having said plasmid vector. The present invention enables realization of a possibility of developing a recombinant crop plant having an acquired resistance against a wide range of pathogenic microorganisms by identifying a complementary DNA encoding a novel chitinase capable of lysing cell walls of both of moulds and bacteria and introducing the sequence thereof into a plant.

Complementary DNA for rice chitinase having lytic activity against

A modified enzyme having a reduced maltopentaose decomposing activity and improvements in practical usability was provided by a gene coding for .alpha.-amylase highly producing maltopentaose, the .alpha.-amylase comprising an amino acid sequence where an amino acid residue at 57- or 139-position has been substituted in the amino acid sequence of maltopentaose-forming amylase derived from Pseudomonas sp. KO-8940 (Shida, O. et al., Biosci. Biotech. Biochem. Vol. 56, 76-80 (1992)).

.alpha.-amylase gene having ability for highly producing maltopentaose,

An object of the present invention is to isolate complementary DNA coding for ornithine carbamyltransferase derived from a rice plant, necessary for development of a plant ornithine carbamyltransferase gene mutated to have phaseolotoxin resistance, and further to establish an expression system in microorganisms for simply evaluating the functions of the gene mutated to have resistance. This object is solved by a rice ornithine carbamyltransferase gene coding for a protein consisting of the amino acid sequence shown in SEQ ID NO:6 in the Sequence Listing, a plasmid vector containing the gene, and a transformant carrying the plasmid vector.

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Tsukuba, Japan

Yoshifumi Ito