Boyce Thompson Institute for Plant Research

Yoshifumi Hashimoto

Robert R Granados

Recent advances of the research on synergistic effect of a mixed baculovirus infection demonstrated the presence of viral molecules enhancing the early event of infection. The enhancins from thewas identified to have such a function, i.e., disrupting the structural integrity of peritrophic membrane of midgut oflarva. The enhancin gene was ligated downstream of the CaMV 355 promoter of a binary vector pBI121. With a drug resistant gene, the gene was introduced to a piece of tobacco leaf, fromcv. Havana SR1. We screened 11 regenerated plants out of 37 by feeding tobacco powder mixed in artificial diet, to 3rd instarlarvae. The larval stage was usually delayed from 1 to 3 days in comparison of that of control larvae. The larvae did not pupate normally. The larvae showed irregular morphology of half larva and half pupae, suggesting a hormonal disturbance caused by the transgenic tobacco. The introduction of an enhancin gene into plants is an effective method of protecting them from insects due to the disruption of their normal life cycle.

Method of protecting plants by introducing a gene coded for a protein which enhances virus infection of host insects

Cloning and sequencing certain baculovirus genes encoding polypeptides termed enhancins. The polypeptides are isolated from the occlusion body of certain baculoviruses such as Trichoplusia ni granulosis virus and Pseudaletia unipuncta granulosis virus, Hawaiian strain. The polypeptides have the ability of enhancing the infectivity of baculoviruses and are useful ingredients of pest control compositions.

Growing community of inventors

Kyoto, Japan

Yoshifumi Hashimoto