Toyota Jidosha Kabushiki Kaisha

Yoshie Takeuchi

Chikara Ohto

Tokuzo Nishino

Shinichi Ohnuma

Hiroyuki Enoki

Keishi Narita

Chika Ishida

Minoru Inamori

Chika Asada

Yuka Higuchi

Manabu Suzuki

When preparing a DNA library via a nucleic acid amplification reaction using a random primer in a convenient and highly reproducible manner, amplification of DNA fragments derived from the chloroplast genome is reduced to a significant extent. A random primer comprises oligonucleotides selected from oligonucleotides group represented by TAAGAGACAGNN excluding those in which 2 bases at the 3' terminus are TG and oligonucleotides group represented by TAAGAGACAGNNN excluding those in which 3 bases at the 3′ terminus are TGC.

Set of random primers and method for preparing DNA library using the same

This invention provides a primer used for a next-generation sequencer that can provide a large number of reads. On the basis of the sequence: 5'-CAAGCAGAAGACGGCATACGAGAT-N-GTCTCGTGGGCTCGGAGATGTGTATAAGA-GACAG-3&#x2032;, wherein Nindicates an index sequence of 5 to 15 nucleotides), an index sequence is designed as a nucleotide sequence exhibiting the putative number of reads, which is calculated using the estimation formula designating the number of reads as a purpose variable and the type of nucleotides in the index sequence as an explanatory variable, exceeding a given level.

Primer for next generation sequencer and a method for producing the same, a DNA library obtained through the use of a primer for next generation sequencer and a method for producing the same, and a DNA analyzing method using a DNA library

Manufacture or use of a mutant prenyl diphosphate synthase in which the amino acid residue located at the fifth position in the N-terminal direction from D of the N-terminal of the aspartic acid-rich domain DDXX(XX)D (the two X's in the parentheses may not be present) present in the second region among the conserved regions of the prenyl diphosphate synthase has been substituted by another amino acid.

Mutant prenyl diphosphate synthase, DNA encoding mutant prenyl diphosphate synthase and process for producing mutant prenyl phosphate synthase

Mutant prenyl diphosphate synthase

DNA coding for thermostable geranylgeranyl diphosphate (GGDP) synthase derived from Sulfolobus acidocaldarius is provided. The DNA is useful for production of GGDP synthase, which is, in turn, useful for production of GGDP.

Geranylgeranyl diphosphate synthase and DNA coding therefor

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Toyota, Japan

Yoshie Takeuchi