Hitachi-high-technologies Corporation

Yoshiaki Sugimura

Toshiro Saito

Ryoji Inaba

Kazumichi Imai

Masatoshi Narahara

Takuya Matsui

The present invention relates to a method for nucleic acid amplification, which enables clusters of amplified nucleic acid fragments to be sequenced by a sequencer to be formed at a high density and improves throughput of nucleic acid sequence analysis by amplifying the number of nucleic acids in the cluster to 10,000 molecules or more; and a method for nucleic acid amplification for enhancing read accuracy, which achieves a high cluster density and increases the number of the amplified fragments in the cluster by the steps of previously forming a pattern of primer DNAs on a base material and fixing bulky template DNA molecules synthesized from DNA samples thereon to induce amplification reaction.

Method and substrate for nucleic acid amplification, and method and apparatus for nucleic acid analysis

Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate (), the nucleic acid having been immobilized on the microparticles () on the substrate () is detected. The microparticles (), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures () aligned on the substrate ().

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Tokyo, Japan

Yoshiaki Sugimura