Yipcell Inc.

Yoo Jun Nam

Ji Hyeon Ju

Yeri Rim

Ye Ri Rim

A method for preparing chondrocyte pellets including: (a) culturing human induced pluripotent stem cells so as to form and obtain embryoid bodies; (b) inducing the embryonic bodies obtained in step (a) to become outgrowth cells and isolating same; and (c) culturing the outgrowth cells isolated in step (b) in a pellet form. A pharmaceutical composition including the chondrocyte pellets prepared by the preparation method; or a method for preventing or treating arthritis, including administering the chondrocyte pellets prepared by the method to an arthritis patient. Chondrocyte pellets of the present invention have a remarkably high rate of differentiation into chondrocytes, uniform size, and a homogeneous differentiation degree, and a pharmaceutical composition, including the pellets, has excellent cartilage regeneration effects at the site of cartilage damage, and is in injectable form, which does not require surgery, such that pain is relieved and a continuous arthritis treatment is provided.

Method for preparing pellets of chondrocytes from human induced pluripotent stem cells, and use thereof

The present invention relates to a method for inducing differentiation, into chondrocytes, of cord blood mononuclear cell-derived induced pluripotent stem cells. In a case where a chondrogenic pellet produced by the method of the present invention is transplanted into a cartilage damage area in vivo, regeneration of cartilage can be effectively exhibited by differentiated chondrocytes. In such a case, an effective cartilage regeneration capacity can be exhibited as compared with a case where chondrocytes produced by differentiation induction with the addition of a recombinant growth factor are transplanted. Thus, the present invention can be usefully used for tissue engineering therapies.

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Bucheon-si, South Korea

Yoo Jun Nam