Monsanto Company

Monsanto Technology LLC

Yonnie S Wu

Jihong Liang

Dilip Maganlal Shah

Cindy Annette Rosenberger

Salim M Hakimi

Jeffrey W Seale

Greg J Bunkers

Cindy A Mittanck

A novel protein was isolated fromand characterized. The protein, termed FCWP1, demonstrated significant antifungal activity against several fungal species. Mutations in proteolytic consensus sequences contained within FCWP1 improved the stability of its antifungal activity. In addition, a class of proteins related to FCWP1 was identified and characterized. This class is made up of ribosomal proteins and displayed similar values for pI and molecular weight. A representative number of proteins from this class were tested and found to have significant antifungal activities. The antifungal proteins disclosed herein are useful in controlling fungal infections in plants. Transgenic plants may be produced that are more resistant to fungal infections relative to non-transgenic plants of the same species. Alternatively, the proteins may be applied to plants exogenously.

Antifungal proteins and methods for their use

Transgenic plants and host cells comprising antifungal polypeptides, isolated from alfalfa plants, are shown to possess antifungal activity. DNA encoding the polypeptides was cloned into vectors for transformation of plant, therby providing a method of inhibiting fungal growth on plants.

Transgenic plants comprising antifungal polypeptides from alfalfa and methods for controlling plant pathogenic fungi

An antifungal polypeptide, AlyAFP, that controls fungal damage to plants is provided. DNA encoding this polypeptide can be cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of inhibiting fungal growth on plants. The polypeptide can be formulated into compositions that can be used to control undesired fungi on plants and elsewhere.

Antifungal polypeptide AlyAFP from Alyssum and methods for controlling plant pathogenic fungi

Antifungal polypeptides, isolated from Medicago plants, are shown to control fungal damage to plants. DNA encoding the polypeptides was cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of inhibiting fungal growth on plants. The polypeptides can be formulated into compositions useful in controlling undesired fungi.

Antifungal polypeptide and methods for controlling plant pathogenic fungi

Antifungal polypeptides, isolated from Medicago plants, are shown to control fungal damage to plants. The polypeptides can be formulated into compositions useful in controlling undesired fungi.

Antifungal polypeptide from alfalfa and methods for controlling plant pathogenic fungi

Isolated DNA molecules encoding an AlyAFP antifungal polypeptide from Alyssum are disclosed. The isolated DNA molecules comprise the nucleotide sequence of SEQ ID NO:12 or encode the amino acid sequence of SEQ ID NO:2. Also disclosed are vectors and transgenic plants comprising said DNA molecules and a method for reducing fungal damage in a plant with said DNA molecule. The isolated DNA molecules may be used to prepare transgenic plants that exhibit reduced damage from fungi as compared to non-transgenic plants. The isolated DNA molecules may be used in combination with additional nucleic acid sequences encoding anti-fungal or anti-insect proteins or polypeptides.

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Vacaville, CA, United States of America

Yonnie S Wu