Tianjin University of Science and Technology

Yongjing Du

Yefu Chen

Xuewu Guo

Dongguang Xiao

Peng Zheng

Guo Zhang

Xiaole Wu

Xinyue Kang

Sen Jiang

Yanrui Ma

Jinying Ren

Astrain with high yield of ethyl butyrate and a construction method and an application thereof are provided. The strain is obtained by over-expressing in the starting strain acetyl coenzyme A acyl transferase gene Erg10, 3-hydroxybutyryl coenzyme A dehydrogenase gene Hbd, 3-hydroxybutyryl coenzyme A dehydratase gene Crt, trans-2-enoyl coenzyme A reductase gene Ter, and alcohol acyl transferase gene AAT. Compared to the starting bacteria not producing ethyl butyrate, the yield of ethyl butyrate of the constructed strain reaches 77.33&#xb1;3.79 mg/L, the yield of the ethyl butyrate of the strain with double copy expression of gene Ter and gene AAT reaches 99.65&#xb1;7.32 mg/L, increased by 28.9% compared with the EST strain, and 40.93&#xb1;3.18 mg/L of ethyl crotonate is unexpectedly produced.

strain with high yield of ethyl butyrate and construction method and application ofstrain

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Tianjin, China

Yongjing Du