Infinitus (china) Company Ltd.

Yonghua Wang

Jian Tang

Dong Liang

Yong Chao Zhou

Zheng Xiao

Zehua Chen

Chenfeng Jing

Provided is a method of producing an oil containing polyunsaturated fatty acids by usingsp. The method comprises: performing fermentation usingsp.; resuspending the resultant cells after fermentation in water, adding cellulase and neutral protease for enzymolysis; mixing the enzymatic hydrolyzate with n-hexane, shaking, extracting, centrifuging, and collecting the n-hexane phase; concentrating the n-hexane phase under reduced pressure to remove the n-hexane, and drying to obtain an oil; performing a first crystallization and a second crystallization, and then performing cold filtration to obtain a liquid oil after the second crystallization; and subjecting the liquid oil to deacidification and decolorization. By regulating fermentation conditions and conducting concentration processing on polyunsaturated fatty acids, the content of eicosapentaenoic acid in thesp. is increased to more than 12%, and the obtained oil is rich in docosahexaenoic acid, docosapentaenoic acid and eicosapentaenoic acid.

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Guangdong, China

Yonghua Wang