Hefei Knature Bio-pharm Co., Ltd.

Dongguan Yutong Optical Technology Co., Ltd.

Yonghong Jin

Wei Wang

Zhanjun Zhang

Feng Tian

Minjie Fu

Zhihao Hu

Chuangbiao Liu

Kanglin Wang

Shilong Mi

Ni Han

Heping Jia

The invention discloses a genetically engineered bacterium in which the gene encoding adenine deaminase on the genome of the bacterium is knocked out or/and the gene encoding the enzyme in the NADanabolic pathway is integrated on the genome of the bacterium. The invention also discloses a construction method of the above-mentioned genetically engineered bacteria. The gene encoding adenine deaminase on the genome of the host strain is knocked out to obtain a strain with high NADyield. Or the expression cassettes of the gene encoding the enzyme in the NADsynthesis pathway are constructed separately, and then the enzyme encoding The gene expression cassette is integrated into the genome of the host strain whose gene encoding adenine deaminase is knocked out to construct a strain with high NADproduction. The application of the above genetically engineered bacteria is disclosed. A method of producing NADis disclosed.

Genetically engineered bacteria, its construction method and its application in producing NADmethod

Growing community of inventors

Anhui, China

Yonghong Jin