Amphastar Pharmaceuticals, Inc.

Other

Nanjing Hanxin Pharmaceutical Technology Co., Ltd.

Amphastar Nanjing Pharmaceuticals Inc.

Bsh Hausgeräte Gmbh

Yonggang Xu

Qi Zhang

Haoning Zhang

Song Chen

Jun Liu

Chuangen Tang

Uwe Dey

Hao Wei

Jundong Meng

Bo Jin

Wencun Wang

Yin Wang

Aoxiang Zhang

Lili Cai

Yongjia Wang

Lijun Chen

Li You

Henrik Birke

Fei Lu

Qixia You

Maoyin Li

Yin Leng

Suiping Zhan

Hui Zhang

Wenfeng Pang

Xiuhua Zhang

The invention relates to a low molecular weight sulfate chondroitin and a preparation method thereof. A low molecular weight chondroitin sulfate with the average molecular weight of less than 1000 Dalton can be obtained by a production process of chondroitin sulfate lyase degradation, deproteinization, filtration and sterilization and drying using macromolecular sulfate chondroitin as a raw material. The low molecular weight Chondroitin sulfate has a narrow molecular weight distribution range, the ratio of chondroitin sulfate disaccharide is 43&#x2dc;60% and the ratio of chondroitin sulfate tetrasaccharide is 30&#x2dc;45%, the sum of chondroitin sulfate disaccharide and chondroitin sulfate tetrasaccharide is more than 87%, the total oligosaccharide content of low molecular weight chondroitin sulfate is more than 97% and the protein content is less than 0.5%; Compared with the general market macromolecule chondroitin sulfate, the product has more remarkable repair effect at the concentration of 50&#x2dc;100 &#x3bc;g/mL on chondrocytes damaged by 1 mM hydrogen peroxide, with strong repair ability and repair rate of 14%&#x2dc;23%. The low molecular weight chondroitin sulfate can be used to treat joint injury and is an important raw material for medical products, health care products, cosmetics and food.

Low molecular weight chondroitin sulfate, composition, preparation method and use thereof

A method of resolving a racemic mixture of (&#xb1;)-Huperzine A to (&#x2212;)-Huperzine A includes: separating the (&#x2212;)-Huperzine A from the racemic mixture of (&#xb1;)-Huperzine A by chiral high performance liquid chromatography (HPLC), the chiral HPLC being performed utilizing a mobile phase including a solution including an alcohol and one selected from dichloromethane, trichloromethane, and a mixture thereof, and the chiral HPLC being performed utilizing a chiral stationary phase including a polysaccharide derivative.

Methods of resolving racemic mixture to obtain (&#x2212;)-Huperzine A

Disclosed is a preparation method for a racemic adrenaline as represented by formula II. The method comprises the following steps: compound 1 is directly racemized in an acidic solution to produce compound 2, the acid solution comprising neither sodium bisulfite nor salicylic acid; and specifically comprises (a), in the acid solution of which the pH is 0.5-1.5, compound 1 is placed under the protection of nitrogen gas and, with the reaction temperature being controlled at 75-95&#xb0; C., stirred and reacted for 1-3 hours; (b) the reaction solution is controlled at a temperature of 5-20&#xb0; C., into which an activated carbon is added, under the protection of nitrogen gas, stirred for 20-40 minutes, and filtered, then a filtrate is collected; the filtrate is controlled at a temperature of 5-20&#xb0; C., the pH thereof is adjusted using ammonia to 8.5-9.5, and is filtered when the pH is stabilized, and a filter cake is washed and dried to produce a high purity racemic adrenaline white powder. The weight yield of the product produced per the preparation method of the present invention is greater than 90%, the chromatographic purity is greater than 96%, and the ee value approaches zero; the invention is inexpensive, simple to operate, favors industrialized production, and has a broad application prospect.

Preparation method for high purity racemic adrenaline

Methods of resolving racemic mixture to obtain (&#x2212;)-huperzine A

A method for the purification of idraparinux sodium includes: passing a solution including a crude idraparinux sodium through a column including a sodium ion exchange resin to obtain a first mixture; passing a solution including the first mixture through a gel chromatogaphy column to obtain a second mixture; and precipitating a purified idraparinux sodium from a solution including the second mixture.

Method of purifying idraparinux sodium

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Nanjing, China

Yonggang Xu