Avi Biopharma, Inc.

Gene Tools, LLC

Brotide Core, LLC

Sarepta Therapeutics, Inc.

Yongfu Li

Dwight D Weller

Matthew Reeves

Christina Mary Josephine Fox

Matthew Dale Reeves

Disclosed is a lipophilic agent comprising a triazine core having lipophilic groups for organic synthesis. The lipophilic agent soluble in one system of solvent(s) wherein the lipophilic agent is participated in a chemical reaction but insoluble by adding a miscible poor solvent to change solution composition after the reaction completes. The lipophilic agent facilitates process improvement wherein practical operation only involves mixing with reactants in solution followed by precipitating with change of solution composition followed by filtering to obtain the precipitated solid, simplifying the purification by isolating the desired solid. The operation is reproducible along the progress in a multi-step synthesis, allowing pure intermediates and pure product as a solid to be rapidly obtained with ease and certainty. This invention can thus accelerate research and development of pharmaceutical biomolecules, representing a tremendous step forward for boosting productivity and greening chemical industry.

Composition of a lipophilic agent for solution phase synthesis of biomolecules

The invention relates to novel fluorescent dyes based on the following pyrenyloxy sulfonamide structure: wherein Ris a leash joined to the pyrenyloxy group via an ether link containing generally a reactive functional group such as, activated carbonate, activated ester, amino group, azide or alkyne for conjugation with biomolecules; Rand Rare hydrogen atoms, or short alkyl chains, or cyclic rings with or without heteroatoms such as nitrogen, oxygen, sulfur, phosphorus. The spectral properties of the fluorescent dyes are sufficiently different in wave-lengths and intensity from fluorescein as to permit simultaneous use of fluorescein and/or more other fluorescent dyes with minimum interference and to avoid interference from endogenous green fluorescent protein in biological system. The dyes are non-ionic to facilitate their entry into cells for intracellular detection. The non-ionic structure also precludes undesired electrostatic reactions with ionic sites on biological components and structures. The dyes have bigger Stokes' shifts than other dyes with similar spectral properties allowing use of simpler, more efficient detection equipment, are not sensitive to pH, and have good solubility in aqueous solution.

Uncharged pyrenyloxy sulfonamide dyes for conjugation with biomolecules

Morpholino compounds are provided having the structure: where

Synthesis of morpholino oligomers using doubly protected guanine morpholino subunits

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Corvallis, OR, United States of America

Yongfu Li