The Hong Kong University of Science and Technology

Nano and Advanced Materials Institute Limited

Siemens Aktiengesellschaft

Yong Yu

Hao Liu

Jing Li

Dan Wang

Benzhong Tang

Wing Yip Lam

Ka Ming Ng

Yang Liu

Ang Li

Jianzhao Liu

Yuning Hong

Sijie Chen

Qian Luo

Tsz Kin Kwok

Wei Qin

Faisal Mahtab

Jianle Zhuang

Nai Wen Tseng

Zhengke Wang

Wentao Hua

Yue Lin Liang

Provided herein are fluorescent bioprobes comprising fluorogens that exhibit aggregation-induced emission (AIE) labeled on biomolecules. The present subject matter relates to a fluorescent bioprobe comprising one or more fluorogen labeled on chitosan. The present subject matter is also directed to methods of preparing the fluorescent bioprobes, methods of labeling and detecting DNA and/or proteins with the fluorescent bioprobe, and methods of cell imaging including live cell tracking.

Aggregation induced emission of fluorescent bioprobes and methods of using the same

Provided herein are magnetic silica fluorescent nanoparticles and fluorescent silica nanoparticles comprising an aggregation induced emission luminogen and magnetite nanoparticles and use of the same as a fluorescent bioprobe for intracellular imaging and a protein carrier. Also provided are processes for preparing and fabricating the same.

Silica nanoparticles with aggregation induced emission characteristics as fluorescent bioprobe for intracellular imaging and protein carrier

Provided herein are a luminogen compound of formula (I) including a AIE luminophore moiety conjugated with a maleimide moiety and a use of the same for detecting thiol groups in biomolecules. Also provided is a dye molecule, a biosensor or a bioprobe comprising the luminogen compound of formula (I) in use for detecting thiol groups in biomolecules. The detection method of the present subject matter not only has high thio-selectivity and sensitivity, but also is rapid, convenient and handy.

Luminogen compounds and the use of the same for biosensing and cellular imaging

Fluorescent bioprobes comprising luminogen formed nanoparticles comprising luminogens with aggregation-induced emission (AIE) properties, which can be used for long-term cell tracking. The luminogens are nonemissive in organic solution but become highly emissive when aggregated in aqueous solution. The fluorescent molecules can readily pass through cell membranes, stain only the cell cytoplasm, and form highly emissive nanoaggregates in aqueous media without any obvious cytoxicity in the living cells. Furthermore, the molecules can be retained inside the cells without noticeable leakage to the outside. Therefore, these AIE-based compounds can be used as selective and cell-compatible fluroescent bioprobes for long-term live cell tracking and imaging.

Aggregation induced emission active cytophilic fluorescent bioprobes for long-term cell tracking

The present subject matter relates to methods for the synthesis of [6,6]-phenyl-C-butyric acid methyl ester (PCBM) and fullerene derivatives in a yield of at least 40%.

Method for synthesis of [6,6]-phenyl-C61-butyric acid methyl ester (PCBM) and fullerene derivatives

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Hong Kong, China

Yong Yu