Sk Chemicals Co., Ltd.

Sk Bioscience Co., Ltd.

Yong Wook Park

Hun Rae Kim

Bong-Yong Lee

Yun-Hee Kim

Hun Sung Kim

Yun Hee Kim

Dong Soo Ham

Hun Kee Kim

Kun Se Lee

Mahnhoon Park

Hwan Ui Jung

Su-Jeen Lee

The present invention relates to a method of preparing an influenza working virus seed stock, a method of increasing infectivity of an influenza working virus seed stock, a method of preparing an influenza vaccine using the seed stock, an influenza vaccine prepared by the method, and an influenza working virus seed stock having increased infectivity.

Method for preparing influenza working virus seed stock, method for preparing influenza vaccine using same seed stock, and virus seed stock prepared by same method

Disclosed is a Madin-Darby canine kidney (MDCK)-derived cell line. The MDCK-derived cell line is derived from MDCK cells deposited under accession number ATCC CCL-34. The MDCK-derived cell line can be prepared by serum-free culture and suspension culture. Preferably, the MDCK-derived cell line has low or no tumorigenicity. The MDCK-derived cell line is preferably selected from MDCK Sky1023, MDCK Sky10234 and MDCK Sky3851. Further disclosed are a culture method for growing the MDCK-derived cells and a method for producing a vaccine virus using the MDCK-derived cells.

MDCK-derived cell lines adapted to serum-free culture and suspension culture and method for preparing vaccine virus using the cells

Growing community of inventors

Suwon-Si, South Korea

Yong Wook Park