E.i. Dupont De Nemours and Company

Yong Tao

Rebecca E Cahoon

Steven Gutteridge

Daniel Cordova

Lihong Wu

Timothy Caspar

James J Rauh

Rejane M Smith

Emil M Orozco, Jr

Wesley B Bruce

Zude Weng

William Dean Hitz

Carl A Maxwell

Jiang-Ming Lee

Timothy Casper

The genes encoding ryanodine receptor homologs have been characterized from multiple insect families. The genes and their corresponding polypeptides have a number of uses including but not limited to the development of screens to identify insecticidally active compounds. Methods are outlined for overcoming toxic effects of expressing recombinant proteins in host cells.

Isolation and use of ryanodine receptors

This invention relates to an isolated nucleic acid fragment encoding a 1-deoxy-D-xylulose 5-phosphate reductoisomerase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase in a transformed host cell.

Plant 1-deoxy-D-xylulose 5-phosphate reductoisomerase

The genes encoding ryanodine receptor homologs have been characterized from multiple insect families including lepidopteran tobacco budworm (), homopteran green peach aphid (), corn plant hopper (), cotton melon aphid () and fruitfly (). The full-length genes have been isolated, cloned and amplified in bacterial cells. Expression in insect cells shows that the recombinant protein folds into a functional calcium release channel. The genes and their corresponding polypeptides have a number of uses including, but not limited to, the isolation of other pest ryanodine receptors, the development of screens to identify insecticidally active compounds, use of fragments of genes as pesticides, fragments of protein for antibody production, fragments of protein for determination of the structure of insecticide binding sites, and identification of insecticides that disrupt the calcium balance in cells through other messengers that interact with the receptor calcium release mechanism. Methods are outlined for overcoming toxic effects of expressing recombinant proteins in host cells.

Ryanodine receptor polypeptides

This invention relates to an isolated nucleic acid fragment encoding an auxin transport protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the auxin transport protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the auxin transport protein in a transformed host cell. The present invention also relates to methods using the auxin transport protein in modulating root development, and in discovering compounds with potential herbicidal activity.

Auxin transport proteins

Nucleic acids encoding ryanodine receptors

This invention relates to an isolated nucleic acid fragment encoding an isopentenyl diphosphate biosynthetic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the isopentenyl diphosphate biosynthetic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the isopentenyl diphosphate biosynthetic enzyme in a transformed host cell.

Rice 1-deoxy-D-xylulose 5-phosphate synthase and DNA encoding thereof

Soybean 1-deoxy-D-xylulose 5-phosphate synthase and DNA encoding thereof

Soybean 1-deoxy-D-xylulose 5-phosphae synthase and DNA encoding thereof

This invention related to an isolated nucleic acid fragment encoding a tryptophan biosynthetic enzyme. This invention also related to the construction of a Chimeric gene encoding all or a portion of the tryptophan biosynthetic enzyme, in sense or antisense orientation, wherein expression of the Chimeric gene results in the production of altered levels of the tryptophan biosynthetic enzyme in a transformed host cell. The tryptophan biosynthetic enzymes include anthranilate phosphoribosyltransferase (E.C. 2.4.2.18) from corn ( ), soybean ( ) and wheat ( ); indole-3-glycerol phosphate synthase (E.C. 4.1.1.48) from corn ( ), rice ( ), soybean ( ), and wheat ( ); and phosphoribosylanthranilate isomerase (E.C. 5.3.1.24) from corn ( ), rice ( ), (and wheat ( ).

Polynucleotides encoding indole-3-gylcerol phosphate synthase from soybean

Growing community of inventors

Newark, DE, United States of America

Yong Tao