Korea Research Institute of Bioscience and Biotechnology

Genkore Co. Ltd.

Republic of Korea

Yong Sam Kim

Jeong Heon Ko

Sun Hee Kim

Jeong Mi Lee

Nan Ee Lee

Su Bin Moon

Dong Hern Kim

Keun Pyo Lee

Yu Jin Kwon

Myong Il Kim

The present disclosure relates to an aglycosylated antibody-producing transgenic mouse and the use of an aglycosylated antibody produced therefrom. When the transgenic mouse of the present disclosure is used, it is possible to easily produce aglycosylated antibodies against various target antigens, and to precisely diagnose disease by detecting a glycoprotein biomarker using the produced aglycosylated antibody.

Transgenic mouse for aglycosylated antibody production and use of aglycosylated antibody produced therefrom

The present invention relates to a composition for genome editing using a CRISPR/Cpf1 system and a use thereof and, more particularly, to a composition for genome editing comprising: a CRISPR RNA (crRNA) including a guide sequence capable of hybridizing with a target nucleotide sequence, and a uridine repeat sequence connected to the 3'-end of the guide sequence, or a DNA encoding the same; and a Cpf1 protein or a DNA encoding the same, a method for genome editing using the same, a method for construction of a genetically modified organism, and a genetically modified organism. The present invention can increase an indel efficiency and decrease off-target activity in genome editing of eukaryotic cells using the CRIPSPR/Cpf1 system and thus can easily construct a genetically modified cell or genetically modified animal or plant having a desired gene inserted thereinto (knock-in) or deleted therefrom (knock-out).

Composition for genome editing using CRISPR/CPF1 system and use thereof

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Daejeon, South Korea

Yong Sam Kim