Monsanto Technology LLC

Yong Huang

Brian J Martinell

Larry A Gilbertson

Michael W Petersen

Xudong Ye

Shihshieh Huang

Edward James Williams

Dennis E McCabe

Qi Wang

Kenneth A Barton

Kenneth John Piller

Toni Armstrong

Laura J Crow

Lori S Julson

Carol A Emler

Christopher G Taylor

Kenneth Barton

The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

Compositions for enhancing segregation of transgenes in plants

A method is disclosed for the-mediated germline genetic transformation of soybean. The method is based on-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.

Soybean transformation method

A novel method is described for the screening of gene elements of interest using hairy roots of chimeric plants transformed with

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Methods for enhancing segregation of transgenes in plants

The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.

Methods for enhancing segregation of transgenes in plants and compositions thereof

A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on an Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, wherein cells are induced directly to form shoots and give rise to transgenic plants. This method does not involve a callus-phase tissue culture and is rapid and efficient.

Soybean agrobacterium transformation method

Growing community of inventors

Madison, WI, United States of America

Yong Huang