Ares Trading S.a.

Prestige Biopharma Pte. Ltd.

Yong Ho Ahn

Sang Kyung Park

Young Min Kim

Dong Heon Lee

Soo Kwang Kim

Sun Kyu Kim

Dae Hae Song

Jung Woo Lee

Ji Yong Yoon

Yu Bin Choi

Byung Je Sung

Jae Young Chang

Eun A Kim

Won Kyum Kim

Yang Soon Lee

Jun Yong Park

Hee Jung Jun

Jae Keun Yu

Hyun Sook Jang

In Hyuk Kim

Hye Soon Won

Myeong Hyeon Jeong

Jung Yim Yim

Jung Tae Lee

Kyo Eun Ahn

Yong Jin Kim

Eun Ho Hwang

Yong Gyu Son

The present invention relates to a bicistronic expression vector for antibody expression, an animal cell transfected with the expression vector, and a method for producing an antibody including culturing the animal cell, in which the expression vector includes a first expression cassette including 'promoter-UTR-intron-antibody light chain gene-polyA' and a second expression cassette including 'promoter-UTR-intron-antibody heavy chain gene-internal ribosome entry site (IRES)-amplification gene-polyA'. An expression vector capable of expressing a desired antibody with high efficiency can be constructed using the bicistronic expression vector including an intron for antibody expression according to the present invention, and the expression vector can produce the antibody by culturing the transfected animal cell with stability and high efficiency.

Bicistronic expression vector for antibody expression and method for producing antibody using same

Provided are a method of preparing an antibody with a regulated, sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content, a method of preparing a high-quality population of antibodies by regulating the sugar content of the antibody to a desired content, and a population of antibodies prepared by the method. Further, provided is a method of regulating the antibody sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content. Furthermore, provided is a medium composition, for regulating the antibody sugar chain content, the medium composition including glycerol as an additive for regulating the antibody sugar chain content. The preparation method of antibodies according to the present invention may be used to prepare a desired high-quality population of antibodies by regulating the sugar chain content of the antibody. Further, in terms of the development of biosimilars, the method of the present, invention may be used to regulate the sugar chain, content of antibodies, thereby preparing antibodies having high equivalence to a control drug. Since the sugar chain content may be regulated by a medium composition, the regulation method is easy and efficient in terms of time and cost, and therefore, widely applied to the fields of antibody preparation.

Method for preparing antibody through regulation of sugar content of antibody

The present invention relates to a method of purifying an antibody with high purity and high quality by removing impurities by sequential use of a cation-exchange column, a culture supernatant multilayer filter and an anion-exchange column without using a protein-A column that is an affinity chromatography column which is generally used for antibody purification.

Method for purifying antibody

The present invention relates to a liquid formulation comprising a TNFR-Fc fusion protein and a stabilizer, in which the fusion protein comprises TNFR (tumor necrosis factor receptor) or a fragment thereof and an immunoglobulin Fc region, and the stabilizer comprises one or more amino acids selected from the group consisting of proline and histidine, a buffer solution, and an isotonic agent containing sodium chloride (NaCl) and sucrose, and a preparation method of the liquid formulation. The liquid formulation according to the present invention provides excellent storage stability because long-term storage of TNFR-Fc fusion protein (etanercept) is possible and particular storage conditions are not needed. Since the liquid formulation of the present invention shows excellent storage stability even though the formulation is simple, it is more economical than other stabilizers or lyophilized formulations, and thus the formulation can be effectively applied for uses wherein treatment of TNFR-Fc fusion protein (etanercept) is beneficial.

Liquid formulation of a fusion protein comprising TNFR and Fc region

The present invention relates to a vector comprising a polynucleotide encoding a modified glutamine synthetase (GS), and a method for preparing a target protein employing the same. More particularly, the present invention relates to a modified GS having an increased sensitivity to a glutamine synthetase (GS) inhibitor, a polynucleotide encoding the modified GS, a vector comprising the polynucleotide, a transformant comprising the vector, and a method for preparing a target protein using the transformant.

Expression vector comprising a polynucleotide encoding a modified glutamine synthetase and a method for preparing a target protein employing the same

The present invention relates to a method for separating and preparing a TNFR-Fc fusion protein using hydrophobic interaction chromatography (HIC). More particularly, the present invention relates to a method for separating and preparing a highly pure active protein from clipped proteins due to displacement effect by adjusting the conductivity of a protein sample using a high concentration of salt solution and by adjusting a loading amount thereof, and a TNFR-Fc fusion protein prepared by the method.

Methods for preparing an active TNFR-Fc fusion protein

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Daejeon, South Korea

Yong Ho Ahn