Olympus Corporation

Yoko Ohashi

Kenji Kawasaki

Kenichi Koyama

Hirobumi Suzuki

Akihiko Yoshikawa

Taro Hayashi

Kiyotsugu Kojima

Seiji Kondo

Shinichi Dosaka

Isao Sakane

Sachiko Karaki

A method for generating a three-dimensional luminescence image includes setting a focal interval between two-dimensional images in accordance with localization of luminescence in a three-dimensional sample. The three-dimensional sample contains a plurality of cells prepared to be luminescent and has a three-dimensional shape. The two-dimensional images have mutually different focal planes and are acquired at the focal interval. The method further includes acquiring a two-dimensional image set including two-dimensional images at the focal interval that is set by imaging the three-dimensional sample under an unirradiated condition; and generating a three-dimensional luminescence image by combining the two-dimensional images included in the two-dimensional image set together.

Method for generating three-dimensional luminescence image, and imaging system

A method for monitoring differentiation into cardiac muscle cells includes keeping, in an alive state, cells into which a reporter gene of luminescent protein configured to vary in luminescence intensity according to an expression of myocardial differentiation marker gene is introduced. The method includes acquiring a luminescence image as a still image by imaging light emitted from the cells in a light shielding state. The method includes acquiring sequential images with illuminating the cells. The method includes associating biological information obtained from the sequential images with biological information obtained from the still image.

Method for monitoring differentiation into cardiac muscle cells

An object of the present invention is to provide a predetermined site luminescence measuring method and a predetermined site luminescence measuring apparatus, which allow for determining whether, when the luminescence from the predetermined site in live samples is measured, a photoprotein is localized at the predetermined site in the same ones as the samples. An predetermined site luminescence measuring apparatusin the present invention is comprised of: a the sampleinto which a fused fusion gene is introduced, the fusion gene being obtained by fusing a fluorescence-related gene that expresses a fluorescence protein in addition to a targeting base sequence and a luminescence-related gene; a containerfor storing the sample, a stageon which the containeris arranged; a luminescent image capturing unitwhich captures a luminescent image of the sample(the objective lensto the CCD camera, and the imaging lens); a fluorescent image capturing unitwhich captures a fluorescent image of the sample(the objective lensto shutter); and an the information communication terminal

Predetermined site luminescence measuring method, predetermined site luminescence measuring apparatus, expression amount measuring method, and measuring apparatus

A fluorescence reader of the present invention detects fluorescence from a sample present on a carrier or in a solution, and the fluorescence reader includes a light source which radiates parallel light, a projection lens which converges the light from the light source, an objective lens which irradiates the sample with the light converged in a rear-side focal position, an image forming lens which forms fluorescence emitted from the sample and passed through the objective lens into an image, a light receiving pinhole disposed in an image forming position of the image forming lens, and detector which detects the fluorescence passed through the light receiving pinhole.

Fluorescence reader wherein uniform fluorescence measurement is accomplished regardless of the state of the measurement object

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Tokyo, Japan

Yoko Ohashi