Ajinomoto Co., Ltd.

Bridgestone Corporation

Yoko Mihara

Yoshinori Tajima

Yosuke Nishio

Yasuo Fukushima

Keiichi Yokoyama

Keita Fukui

Kunio Nakata

Hiroaki Rachi

Saaya Takaoka

Yosifovna Joanna Katashkina

Yasuyuki Hayashi

Dmitrievna Ekaterina Kazieva

Minako Harada

Yohei Tatsukami

Georgievna Irina Andreeva

The present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, productivity of which has been enhanced. Specifically, the present invention provides a method of producing 13-hydroxy-9(Z)-octadecenoic acid, by producing 13-hydroxy-9(Z)-octadecenoic acid from linoleic acid in the presence of a transformed microorganism that produces a protein such as the following: (A) a protein having an amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14; (B) a protein having an amino acid sequence containing one or several amino acid substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13, or 14, and having a linoleate 13-hydratase activity; and (C) a protein having an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NOs: 4, 5, 8 to 10, 13 or 14, and having a linoleate 13-hydratase activity.

Method of producing 13-hydroxy-9(Z)-octadecenoic acid

A host cell includes a heterogeneous expression unit including: (a) a polynucleotide encoding a mevalonate kinase derived from a microorganism belonging to a genus selected fromandand (b) a promoter operatively linked to the polynucleotide. The host cell is used to produce mevalonate kinase, mevalonate-5-phosphate, and isoprenoid compounds.

Method of producing isoprene monomer

An organic acid is produced by allowing a bacterium which has an ability to produce an organic acid and has been modified so that expression of the sucE1 and mdh genes are enhanced, or a product obtained by processing the bacterium, to act on an organic raw material in a reaction mixture containing carbonate ions, bicarbonate ions, or carbon dioxide gas, and collecting the organic acid.

Method for producing an organic acid

The present invention provides means useful for establishing an excellent isoprene monomer production system. Specifically, the present invention provides a polynucleotide of the following (a), (b), or (c):

Isoprene synthase and gene encoding the same, and method for producing isoprene monomer

Growing community of inventors

Kawasaki, Japan

Yoko Mihara