The University of Tokyo

Thinkcyte, Inc.

Osaka University

Bristol-myers Squibb Compnay

Thinkcyte K.k.

Yoko Kawamura

Sadao Ota

Masashi Ugawa

Ryoichi Horisaki

Issei Sato

Masaaki Nagase

Teruhisa Kaneko

Keiji Nakagawa

Yuka Shinohara

The present disclosure provides methods and systems for ghost cytometry (GC), which may be used to produce an image of an object without using a spatially resolving detector. This may be used to perform image-free ultrafast fluorescence 'imaging' cytometry, based on, for example, a single pixel detector. Spatial information obtained from the motion of cells relative to a patterned optical structure may be compressively converted into signals that arrive sequentially at a single pixel detector. Combinatorial use of the temporal waveform with the intensity distribution of the random or pseudo-random pattern may permit computational reconstruction of cell morphology. Machine learning methods may be applied directly to the compressed waveforms without image reconstruction to enable efficient image-free morphology-based cytometry. Image-free GC may achieve accurate and high throughput cell classification as well as selective sorting based on cell morphology without a specific biomarker, which have been challenging using conventional flow cytometers.

Methods and systems for cytometry

Cytometry system and method for processing one or more target cells from a plurality of label-free cells

The present disclosure provides systems, devices, and methods for flow focusing in a microfluidic device. Flow focusing may be used in detection of objects, for example cells, in a stream of fluid passing through a fluidic device. The systems and devices may comprise a flow channel positioned between two sheath channels configured to direct fluid across the flow channel. Flow focusing microfluidic systems and devices disclosed herein may be robust to alignment errors. Systems and devices of the present disclosure may reduce the displacement of flow from the intended locations due to alignment errors. Also disclosed herein are methods for using such microfluidic systems and devices.

Microfluidic devices and uses thereof

An imaging flow cytometer includes at least one flow channel through which an observation target flows, a light source which irradiates the flow channel with sheet-like excitation light, an imaging unit which images a specific cross-section of the observation target by imaging fluorescence from the observation target having passed through a position irradiated with the excitation light, and a three-dimensional image generation unit which generates a three-dimensional image of the observation target as a captured image on the basis of a plurality of captured images obtained by cross-sectional imaging by the imaging unit.

Imaging flow cytometer

A cell evaluation system includes physical measurement unit, a database, and evaluation unit. The evaluation unit refers to a relevance stored in the database, searches reference measurement information based on measurement information of a cell newly measured via the physical measurement unit, and evaluates the cell with biological measurement information associated with the searched reference measurement information.

System and method for cell evaluation, and cell evaluation program

The present invention relates to an anti-inflammatory and analgesic pharmaceutical preparation for external use having excellent percutaneous absorption and applicability. The pharmaceutical preparations for external use of this invention comprise NSAIDs and, as a percutaneous absorption promoting agent, oleic acid, oleyl alcohol or a mixture thereof, in a pharmaceutically acceptable aqueous alcoholic solvent comprised of a monohydric saturated aliphatic alcohol of 1-4 carbon atoms, a polyhydric alcohol selected from the group consisting of saturated aliphatic glycols of 2-4 carbon atoms and glycerol, and water.

Pharmaceutical preparations for external use containing non-steroidal anti-inflammatory and analgesic agents

Growing community of inventors

Tokyo, Japan

Yoko Kawamura