Corning Incorporated

Public University Corporation Yokohama City University

Kuraray Co., Ltd.

Yoko Itchoda

Hideki Taniguchi

Motohiro Fukuda

Go Tazaki

Hitoshi Tsuruta

Keisuke Sekine

Masaya Hosoda

Yun-Wen Zheng

To provide a cell culture kit including cultured living cells of various donors, and a manufacturing method thereof. The cell culture kit includes a culture plate and living cells cultured thereon. The culture plate includes a plurality of microchambers () and living cells derived from various donors are adhered to surfaces of the plurality of microchambers (). Specifically, living cells D, D, and Dderived from various donors are adhered to the plurality of microchambers (). In each microchamber (), living cells derived from one donor or living cells derived from various donors may be cultured. The living cells derived from various donors are adhered and cultured in the cell culture kit as a whole, which makes it possible to provide a cell culture kit to conduct a test using cells derived from various donors.

Cell culture kit, screening method, and method of manufacturing cell culture kit

Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid body size is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culture chamber () having a plurality of microchambers () formed on a culture surface. The cell culture chamber () has a culture surface formed of spaces in which the microchambers () have a space structure with a height of 10 &#x3bc;m to 500 &#x3bc;m and a bottom area of 100 &#x3bc;mto 1 mm. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammalian cells to obtain a cell population at least partially differentiated into endoderm lineage cells, by using the cell culture chambers ().

Method for differentiating pluripotent mammalian stem cells into a population of hepatic cells in a microchamber

Growing community of inventors

Tsukuba, Japan

Yoko Itchoda