Riken Corporation

The Institute of Physical and Chemical Research

Riken Genesis Co., Ltd.

Yoko Aida

Shin-nosuke Takeshima

Susumu Saito

Mayuko Tsunoda

Mikio Kawahara

Yuri Muramatsu

Yuan Yuan

Mayuko Jimba

Daiji Endoh

Masakazu Kamata

Shin-Nosuke Takeshima

A forward primer of a primer set in accordance with the present invention for detecting BLV is a mixture of (1) a first primer consisting of a polynucleotide including 15 or more successive bases including the 16th C in the base sequence of SEQ ID NO: 1 and (2) a second primer consisting of a plurality of kinds of polynucleotides including at least the first to 15th bases in the base sequence of SEQ ID NO: 2. Two or more of M, N, Y, K, and D which are included in the second primer are each a degenerate base which specifies two or more kinds of bases, and the second primer includes at least 10 kinds of polynucleotides including at least the first to 15th bases in the base sequences of SEQ ID NOs: 3 to 12.

Primer set for detecting bovine leukemia virus and use thereof

A kit for detecting Bovine leukemia virus (BLV) according to the present invention includes: a first PCR primer being oligonucleotide including successive 20 bases or more in the base sequence denoted by SEQ ID NO: 1, the first PCR primer being oligonucleotide having 50 bases or less; and second PCR primer being including successive 20 bases or more in the base sequence denoted by SEQ ID NO: 2, the second PCR primer being oligonucleotide having 50 bases or less is included.

Kit for detecting bovine leukemia virus(BLV), and use thereof

An apoptosis inducing agent which comprises a protein as being Vpr protein encoded by vpr gene of HIV-1 wherein 15 amino acid residues from the C-terminal are deleted, and an apoptosis inducing gene encoding said protein. The agent induces apoptosis of cells and therefore useful as a medicament for treatment of a cancer or AIDS.

VPR mutant protein and its encoding gene having apoptosis-inducing action

A c143 monoclonal antibody which is used for detecting a bovine individual having a possibility of onset of bovine leukemia; a monoclonal antibody which is used for detecting a bovine individual having a possibility of onset of bovine leukemia, wherein the monoclonal antibody has the substantially same reactivity as the c143 monoclonal antibody to a bovine MHC Class II DR molecule to which a possibility of onset of bovine leukemia is attributable; and an agent for diagnosing a possibility of onset of bovine leukemia which comprises the aforementioned monoclonal antibody. Bovine individuals having a possibility of onset of bovine leukemia can be conveniently and accurately detected.

Antibodies for judging possibility of the onset of bovine leukemia

Method for judging the possibility of the onset of sheep leukemia

A method for judging a possibility of the onset of bovine leukemia caused by bovine leukemia virus BLV, wherein a bovine individual, in which an amino acid sequence defined by the amino acid numbers 75 to 78 of the &bgr;1 domain of the bovine MHC Class II DR&bgr; chain is Val-Asp-Thr-Tyr, is judged to have a possibility of the onset of the leukemia; and a method for judging a resistance to the onset of bovine leukemia caused by the bovine leukemia virus BLV, wherein a bovine individual, in which an amino acid defined by the amino acid number 78 of the &bgr;1 domain of the bovine MHC Class II DR&bgr; chain is Val, is judged to have a resistance to the onset of the leukemia.

Methods for judging the possibility of the onset of bovine leukemia and the resistance thereto

Methods for judging the possibility of the onset of bovine leukemia and

Growing community of inventors

Ibaraki, Japan

Yoko Aida