Agency for Science, Technology and Research

Yoke San Daniel Lee

Jackie Yi-Ru Ying

Guolin Xu

Min-Han Tan

Igor Cima

Yeon Joon Park

Wai Min Phyo

Tseng-Ming Hsieh

Emril Mohamed Ali

Mo-Huang Li

Jackie Y Ying

A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.

Column-based device for retrieval of rare cells based on size, and uses thereof

Column-based device and method for retrieval of rare cells based on size, and uses thereof

According to various embodiments, a reagent fluid dispensing device may be provided. The reagent fluid dispensing device may include a chamber for receiving a reagent fluid, the chamber having a first opening and a second opening; a first fluid conduit connected to the first opening of the chamber; a reservoir connected to the first fluid conduit, the reservoir having a first opening, wherein the first opening of the reservoir is connected to the first fluid conduit to form a passive valve, wherein the reservoir is dimensionalized for storing a predetermined volume of the reagent fluid; and a pneumatic conduit connected to the second opening of the chamber, wherein selective application of pneumatic pressure to the chamber through the pneumatic conduit transfers the reagent fluid from the reservoir to the chamber through the first fluid conduit. According to various embodiments, a microfluidic device including the reagent fluid dispensing device, and a method of dispensing a reagent fluid may be provided.

Reagent fluid dispensing device, and method of dispensing a reagent fluid

A microfluidic separation system, which comprises a magnetic separator, which itself comprises a magnetic energy source; first and second magnetically conductive members leading from the magnetic energy source and having respective terminal ends that are separated by a gap over which a magnetic field is applied due to the magnetic energy source. The separation system further comprises a microfluidic chip for insertion into the gap, which comprises a body defining channels on respective faces of the body; and an exterior lining that seals the plurality of channels to allow separate test sample volumes to circulate in at least two of the channels. Upon insertion of the chip into the gap, a first test sample volume is confined to circulating closer to the terminal end of the first member and a second test sample volume is confined to circulating closer to the terminal end of the second member.

Microfluidic separation system

Growing community of inventors

Singapore, Singapore

Yoke San Daniel Lee