Kyoto University

Kirin Holdings Kabushiki Kaisha

Takeda Pharmaceutical Company Limited

Yohei Kawai

Shin Kaneko

Shinichiro Takayanagi

Akira Hayashi

Yoshiaki Kassai

Ken Fukumoto

Kazuki Nakazono

Atsushi Kunisato

Sayaka Chuganji

Suguru Arima

Kazuhide Nakayama

Maiko Takiguchi

The present invention relates to a method for producing T cells or NK cells including culturing T cells or NK cells in a culture medium containing a CaMKII inhibitor such as a bisbenzylisoquinoline alkaloid compound. The disclosed compounds and methods can be used for maintaining the undifferentiated state of undifferentiated T cells, or for efficiently proliferating T cells or NK cells.

Production method for T cells or NK cells, medium for culturing T cells or NK cells, method for culturing T cells or NK cells, method for maintaining undifferentiated state of undifferentiated T cells, and growth-accelerating agent for T cells or NK cells

A method efficiently produces cytotoxic T lymphocytes having intrinsic properties of lymphocytes of the acquired immune system suitable for cellular immunotherapy. The method includes culturing CD4/CD8 double-positive T cells in a medium containing IL-7 and a T-cell receptor activator, to induce CD8αβcytotoxic T lymphocytes.

Method for producing CD8alpha+beta+ cytotoxic t cells

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Kyoto, Japan

Yohei Kawai