National Pingtung University of Science & Technology

Institute of Nuclear Energy Research, Atomic Energy Council, Executive Yuan

Biomixin Co., Ltd.

Yo-Chia Chen

Yu-Chuan Chiang

Hsueh-Ling Cheng

Wen-Song Hwang

Gia-Luen Guo

Jia-Baau Wang

Teng-Chieh Hsu

Rung-Sheng Peng

Jeng-Chen Liu

Hau-Yu Wang

A fusant F001 able to digest polysaccharides is formed by protoplast fusion betweenand. The fusant F001 is deposited at NITE Patent Microorganisms Depositary (NPMD) in Japan with a deposit number NITE BP-02873.

Fusant F001 able to digest polysaccharides

A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed withsp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred toby transformation, such thatcan acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.

Cellulose hydrolase and gene thereof

An isolated xylanase gene with mutations includes a fifty-eighth amino acid or a thirty-eighth amino acid generated from transforming asparagine to aspartic acid so as to form the isolated xylanase gene. A site-specific mutagenesis method includes: mutating the forty-first amino acid or the thirty-eighth amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the isolated xylanase gene.

Isolated xylanase gene with mutations and site-specific mutagenesis method thereof

A mutated xylanase gene with a broad pH range of reaction includes a forty-first amino acid or a twenty-first amino acid generated from transforming asparagine to aspartic acid so as to form the mutated xylanase gene. A site-specific mutagenesis method includes the step of: mutating the forty-first amino acid or the twenty-first amino acid of the xylanase gene by transforming asparagine to aspartic acid so as to form the mutated xylanase gene.

Growing community of inventors

Pingtung County, Taiwan

Yo-Chia Chen