Hvidovre Hospital

Københavns Universitet

Kobenhavns Universitet

Yiping Li

Jens Bukh

Judith Margarete Gottwein

Santseharay Ramirez Almeida

Troels Kasper Hoyer Scheel

Tanja Bertelsen Jensen

Troels Kasper Høyer Scheel

Jannick Prento

Jacob Bo Lademann

Daryl Grant Humes

The present invention relates to nucleic acid sequences that encode hepatitis C viruses (HCV) that are useful in the fundamental research of HCV as well as in the search of a vaccine against HCV. In particular the present invention relates to nucleic acid sequences that comprises HCVs which are capable of expressing said virus when transfected into cells and are capable of infectivity in vivo.

Optimized HCV full-length infectious cell culture systems and applications thereof

The present invention relates to hepatitis C virus (HCV) culture systems of genotypes 1a, 3a, 4a, 5a, and 6a that directly contribute to HCV drug and vaccine development, to HCV basic research and better-individualized treatment of HCV infected patients.

HCV culture systems and direct-acting antiviral sensitivity

The present invention relates to nucleic acid sequences that encode hepatitis C viruses (HCV) of genotype 2b that are useful in the fundamental research of HCV as well as in the search of a vaccine against HCV. In particular the present invention relates to nucleic acid sequences that comprises HCVs, which are capable of expressing said virus when transfected into cells and are capable of infectivity in vivo.

High-titer HCV full-length genotype 2B infectious cell culture systems and applications thereof

HCV full-length infectious cell culture systems and applications thereof

Hepatitis C reporter viruses containing Core through NS2 of prototype isolates of all major HCV genotypes and the remaining genes of isolate JFH1, by insertion of reporter genes in domain III of HCV NS5A were developed. A deletion upstream of the inserted reporter gene sequence conferred favorable growth kinetics in Huh7.5 cells to these viruses. These reporter viruses can be used for high throughput analysis of drug and vaccine candidates as well as patient samples. JFH1-based intergenotypic recombinants with genotype specific homotypic 5'UTR, or heterotypic 5&#x2032;UTR (either of genotype 1a (strain H77) or of genotype 3a (strain S52)) were also developed. The present inventors additionally developed J6/JFH1 recombinants with the 5&#x2032;UTR of genotypes 1-6. These recombinants with different 5&#x2032;UTRs are a useful to study the function of the 5&#x2032;UTR in a genotype specific manner.

Hepatitis C virus expressing reporter tagged NS5A protein

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Hvidovre, Denmark

Yiping Li