Ningbo University

Yingying Wei

Yi Chen

Xingfeng Shao

Wenhao Li

Lijian Ding

Shu Jiang

Weiyan Zhang

Yihui Mao

Shan He

Disclosed are an antimycin compound and a preparation method and use thereof. The preparation method comprises: fermenting a marine actinomycete (sp.4-7) with a preservation number CCTCCNO: M2020953 to obtain a fermented product and soaking and extracting the fermented product with ethyl acetate to obtain a crude extract; and carrying out separation and purification by normal-phase silica gel column chromatography, reversed-phase MPLC, and semi-preparative reversed-phase high-performance liquid chromatography. The invention has the advantages of resistance againstand

Method for preparing an antimycin compound produced bysp.4-7

The present invention discloses apolygalacturonase-inhibiting protein 1 (PpPGIP1) gene, and a cloning method and use thereof. Thepolygalacturonase-inhibiting protein 1 PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by thepolygalacturonase-inhibiting protein 1 (PpPGIP1) gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product.

polygalacturonase-inhibiting protein 1 (PpPGIP1) gene, and cloning method and use thereof

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Zhejiang, China

Yingying Wei