University of California

Pioneer Hi-bred International, Inc.

Yinglei Tao

Hemantha Kumar Wickramasinghe

Abraham Phillip Lee

Xuan Li

Do-Hyun Lee

Yue Yun

H Kumar Wickramasinghe

Disclosed is a lab-on-a-chip platform comprising a microfluidic device sealed with a thin membrane. An analysis probe configured to penetrate the thin membrane to access a sample trapped in the microfluidic device.

Integrated microfluidic platform for selective extraction of single-cell mRNA

Methods of non-destructively obtaining the genotype of a plant cell from a plant sample are disclosed. The plant cell is isolated from a plant cell sample using an integrated microfluidic device. The integrated microfluidic device includes an individual cell trap located downstream from a microfluidic channel and, the microfluidic device is configured to trap the plant cell in the individual cell trap. mRNA is extracted from the plant cell by contacting the plant cell with an atomic force microscope (AFM) probe and by attracting mRNA from loci of interest to the probe end using a dielectrophoresis DEP force under the alternating current (AC) field applied to the probe. The genotype of the plant cell is determined from cDNA obtained from the extracted mRNA. Alternatively, the mRNA is analyzed to determine gene expression patterns of the plant cell.

Single cell microfluidic device

Growing community of inventors

Irvine, CA, United States of America

Yinglei Tao