Mcmaster University

Yingfu Li

John D Brennan

Carlos Filipe

Sergio David Aguirre

Leyla Soleymani

Robert Pelton

Todd Hoare

Meng Liu

Sana Jahanshahi-Anbuhi

M Monsur Ali

Meghan Rothenbroker

Erin M McConnell

Dingran Chang

Michael Gregory Wolfe

Sahar Esmaeili Samani

Carmen Carrasquilla

Yang Lu

Shirley Mei

Zhongjie Liu

Razvan Nutiu

Monsur Ali

Richa Pandey

Ravan Nutiu

The present application is directed to methods of performing chemical reactions, including multi-step chemical reactions in which two or more of the reagents in the chemical reaction are incorporated or entrapped in a solid polymeric structure comprising pullulan. In certain embodiments, the chemical reaction or multi-step reaction serves as a sensor. Accordingly the present application is also directed to sensors for performing the methods of the application. In certain embodiments, at least one of the reagents is a biomolecule and the sensor is a biosensor. In certain other embodiments, the solid polymeric structure comprising pullulan and the reagents for performing a chemical reaction form a convenient device for performing a chemical reaction.

Method of stabilizing molecules without refrigeration using water soluble polymers and applications thereof in performing chemical reactions

This disclosure relates to biosensors, and in particular, biosensors based on nucleic acid cleaving enzymes such as ribonucleotide-cleaving DNAzymes for the detection of analytes, and methods of use.

Nucleic acid cleaving enzyme-based biosensor and methods of use thereof

Provided herein are nucleic acid-cleaving catalytic nucleic acid probes, biosensors and lateral flow biosensor devices and methods and kits of using the probes, biosensors and lateral flow biosensor devices for detecting an analyte present on or generated from a microorganism in a test sample, includingand methods for determining whether a subject has ainfection.

DNAzyme-based sensor for

The present application is directed to biosensors and methods for detecting a microorganism target in a sample using a mechanically interlocked nucleic acid catanane, wherein an enzyme from the microorganism target or that is activated by a molecule from the microorganism target cleaves a linkage in a first single-stranded nucleic acid ring of the catanane structure, allowing rolling-circle amplification to occur and the presence of rolling-circle amplification products indicates the presence of the microorganism in the sample.

Nucleic acid catenane with a linking duplex biosensor for detection of a microorganism target

Provided herein are aptamers, aptamer probes and biosensor systems that detectglutamate dehydrogenase (GDH). Also provided are methods of detectingGDH using the aptamers, probes and biosensors and methods of determining whether a subject has ainfection.

Aptamers for clostridium difficile detection

The present application discloses a biosensor that comprises a nucleic acid probe absorbed on reduced graphene oxide, the nucleic acid probe comprising an RCA primer sequence linked to a recognition moiety for an analyte to be detected by the biosensor.

Reduced graphene oxide-based biosensors

The present application relates to biosensors, for example comprising an high-molecular weight, tandem repeating, structure-switching nucleic acid aptamers (concatemeric aptamers) to rapidly create patterned sensors via, for example, inkjet printing. These concatemeric aptamer reporters remain immobilized at the point of printing through strong adsorption but retain sufficient segmental mobility to undergo structure switching and reporter signalling to provide both qualitative and quantitative detection of one or more analytes. In certain embodiments, inkjet printing allows for the patterning of internally referenced sensors with multiplexed detection, and provides a generic platform for on-demand printing of sensors even in remote locations.

Biosensors comprising concatemeric nucleic acid molecules

The disclosure relates to a method of generating catalytic nucleic acid probes useful for detecting microorganisms such as bacterial pathogens. In one embodiment, the catalytic nucleic acid probes are fluorogenic DNAzymes. The disclosure also relates to catalytic nucleic acid probes and methods of using the probes for detecting microorganisms.

Catalytic nucleic acid probes as microbial indicators

Methods for the selection of novel signaling allosteric DNA enzymes are provided. In particular, fluorescent signaling allosteric DNA enzymes are described. The selection system is based on the cleavage of an ribonucleotide flanked by a fluorophore modified nucleotide and a quencher modified oligonucleotide. Both cis-acting and trans-acting allosteric DNA enzymes are identified, as well as aptamer/DNA enzyme conjugates.

DNA enzymes

The present invention provides a method for the selection of aptamer sequences and aptamers identified using the method. The method comprises immobilizing a library oligonucleotide by forming a duplex with an antisense oligonucleotide, reating the duplex molecule with a target and collecting oligonucleotide molecules which dissociate from the duplex structure.

Growing community of inventors

Dundas, Canada

Yingfu Li