Chinese Academy of Sciences

Other

Yingfang Liu

Jie Zhou

Zihe Rao

Zonghao Zeng

Huanhuan Liang

Juan Shen

Xiaojing He

Zhikai Xiahou

The present invention relates to methods and compositions for treating and/or preventing a disease or disorder associated with abnormally high level of the IFP35 family of proteins, including IFP35 and NMI, methods and compositions for diagnosis, prognosis or treatment monitoring of a disease or disorder associated with abnormally high level of the IFP35 family of proteins, including IFP35 and NMI, and methods and compositions for identifying a modulator of the IFP35 family of proteins, including IFP35 and NMI.

Methods and compositions for treating and/or preventing a disease or disorder associated with abnormal level and/or activity of the IFP35 family of proteins

Present invention discloses the three-dimensional crystal structure of the N-terminus polypeptide of influenza virus polymerase subunit (PA_N of SEQ ID NO:7). PA_N of SEQ ID NO: 1 is residues 1&#x2014;50 to 150-300 of influenza virus polymerase subunit PA. In the three-dimensional structure, at least 40% of atoms show the same atomic coordinates, compared to that listed in Table 1. Namely, in the three-dimensional structure of influenza virus polymerase subunit PA_N of SEQ ID NO: 7, 40% of atomic coordinates on carbon skeleton of residues of influenza virus polymerase subunit PA_N of SEQ ID NO: 7, show less than or equal to 1.7 &#x212b; of average variance, compared to the atomic coordinates listed in Table 1. Present invention also discloses the expression, purification, crystallization methods, and three-dimensional crystal structure of 256 residues in the N-terminus of influenza virus polymerase subunit PA, and applications of the crystal structure of SEQ ID NO: 7 on drug screening and designing.

Crystal structure of amino terminal portion of influenza virus polymerase PA subunit and use thereof

Provided are the expression method of influenza virus polymerase PAc-PB1complex, the co-crystallization method of the complex and the three-dimendional structure of the crystal of PAc-PB1complex. Also provided are the compounds binding to the influenza virus polymerase PAc and the expression method of influenza virus polymerase PA. The three-dimensional structure of the crystal of PAc-PB1complex can be used for screening and designing the drug for the treatment of influenza.

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Beijing, China

Yingfang Liu