Battelle Memorial Institute

Clemson University

Gaa Custom Engineering LLC

Ying Zhu

Ryan T Kelly

Richard Dale Smith

Gordon A Anderson

R Kenneth Marcus

David W Koppenaal

Tao Liu

Kristin E Burnum-Johnson

Ronald J Moore

Edward D Hoegg

Chris Anderson

Tyler Williams

Paul D Piehowski

Chia-Feng Tsai

Charles K Ansong

Geremy Cd Clair

Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation.

Nanoscale biochemical sample preparation and analysis

Provided herein are methods and systems for proteome analysis that are at least partially automated and/or performed robotically. In some aspects, the methods and systems described herein can rapidly and efficiently provide protein identification of each of the proteins from a proteome, or a complement of proteins, obtained from extremely small amounts of biological samples. The identified proteins can be imaged quantitatively over a spatial region. Automation and robotics facilitates the throughput of the methods and systems, which enables protein imaging and/or rapid proteome analysis.

Methods and systems of proteome analysis and imaging

Apparatus include an atmospheric pressure glow discharge (APGD) analyte electrode defining an analyte discharge axis into an APGD volume, and a plurality of APGD counter electrodes having respective electrical discharge ends directed to the APGD volume, wherein the APGD analyte electrode and the APGD counter electrodes are configured to produce an APGD plasma in the APGD volume with a voltage difference between the APGD analyte electrode and one or more of the AGPD counter electrodes. An electrode can be integrated into an ion inlet. Apparatus can be configured to perform auto-ignition and/or provide multi-modal operation through selectively powering electrodes. Electrode holder devices are disclosed. Related methods are disclosed.

Multi-electrode/multi-modal atmospheric pressure glow discharge plasma ionization device

Growing community of inventors

Richland, WA, United States of America

Ying Zhu