Canon U.S. Life Sciences, Inc.

Ying-Xin Wang

Kenton C Hasson

Johnathan Stuart Coursey

Brian P Murphy

Eric D Schneider

Hiroshi Inoue

Shulin Zeng

Ben Lane

Alex K Flamm

Ivor T Knight

Gregory H Owen

Scott Corey

Weidong Cao

Hongye Liang

Sami Kanderian

Conrad Laskowski

Brian Bean

Takayoshi Hanagata

Franklin Regan

Jason Schmidt

The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

System and method for serial processing of multiple nucleic acid assays

The present invention relates to compositions and methods for in-system priming of microfluidic devices. Aspects of the present invention relate to compositions and methods for in-system priming of microfluidic devices utilizing a priming solution comprising adding and/or increasing concentrations of surfactant in a buffer solution.

Composition and method for in-system priming microfluidic devices

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Rockville, MD, United States of America

Ying-Xin Wang