Omniome, Inc.

Pacific Biosciences of California, Inc.

Ying Lin Liu

Pinar Iyidogan

Mark C Wallen

Arnold Oliphant

Brittany Ann Rohrman

Fabian Block

Morassa Mohseni Middleton

Michael James Schmidt

Kandaswamy Vijayan

Methods, compositions, kits and apparatuses that include a fluid, the fluid containing a ternary complex and Li, wherein the ternary complex includes a primed template nucleic acid, a polymerase, and a nucleotide cognate for the next correct base for the primed template nucleic acid molecule. As an alternative or addition to Li, the fluid can contain betaine or a metal ion that inhibits polymerase catalysis such as Ca. In addition to Li, the fluid can contain polyethylenimine (PEI) with or without betaine.

Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes

Provided are engineered DNA polymerases exhibiting modified functionality, and polynucleotides encoding same. Modified features include: (1) reduced catalytic activity in the presence of magnesium ions and/or (2) reduced affinity for primed template nucleic acid molecules in the absence of cognate nucleotide, and an ability to discriminate between cognate and non-cognate nucleotides under low salt conditions. Sequencing By Binding&#x2122; procedures employing the engineered polymerases have certain advantages. The engineered polymerases can have other uses as well.

Polymerases engineered to reduce nucleotide-independent DNA binding

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San Diego, CA, United States of America

Ying Lin Liu