Bristol-myers Squibb Compnay

Abbvie Inc.

Ultragenyx Pharmaceutical Inc.

Ying Jing

Zhengjian Li

Yueming Qian

Zehra Kaymakcalan

Christopher Chumsae

Xiaobei Zeng

Kartik Subramanian

Chee Furng Wong

Mayda Perez Thiele

Jan Panteli

The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise the addition of glucocorticoid compound during the culturing period. The addition of glucocorticoid compound sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein.

Mammalian cell culture processes for protein production

The instant invention relates to modulated lysine variant species compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and/or protein purification methods, for producing such modulated lysine variant species compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNF&#x3b1; is detrimental, are also provided.

Modulated lysine variant species compositions and methods for producing and using the same

Growing community of inventors

Wellesley, MA, United States of America

Ying Jing